2型糖尿病小鼠周围神经病变模型的建立及皮下神经纤维密度的检测  

Establishing mouse model of type 2 diabetes peripheral neuropathy and measuring its intra-epidermal nerve fiber density

作　　者：浦江畔 唐佳雯[1] 朱涛[1] PU Jiangpan TANG Jiawen ZHU Tao.(Shanghai Songiiang district center hospital affiliated to Nanjing Medical University, Shanghai 201600, China)

机构地区：[1]南京医科大学附属上海市松江区中心医院麻醉科,201600

出　　处：《临床麻醉学杂志》2017年第10期1006-1010,共5页Journal of Clinical Anesthesiology

基　　金：上海市科委医学引导项目基金资助(134119b2700)

摘　　要：目的建立2型糖尿病小鼠周围神经病变模型,并对该模型的皮下神经纤维密度(intra-epidermal nerve fiber density,IENFD)进行检测。方法 C57BL6雄性小鼠24只,随机分为四组,每组6只:HS组采用高脂饲料喂养24周+链脲佐菌素STZ(120 mg/kg)单次注射;H组采用高脂饲料喂养24周+缓冲液注射;S组普通饲料喂养24周+STZ(120 mg/kg)单次注射;C组普通饲料喂养24周+缓冲液注射。四组小鼠均以24周为实验终点,测定随机血糖、胰岛素抵抗指数(HOMA-IR)、机械痛阈和IENFD。结果第24周时HS组随机血糖、HOMA-IR明显高于H、S、C组(P<0.01),机械痛阈明显低于H、S、C组(P<0.05),IENFD明显低于S组和C组(P<0.05);H组随机血糖、HOMA-IR明显高于C组(P<0.01),机械痛阈与S组、C组差异无统计学意义,IENFD与HS组差异无统计学意义。结论高脂饲料喂养联合中等剂量STZ成功建立了2型糖尿病小鼠周围神经病变模型,小鼠皮下神经纤维密度明显下降。Objective To establish mouse model of type 2 diabetes peripheral neuropathy and measure its intra-epidermal nerve fiber density（IENFD）.Methods Male C57BL6 mouse were randomly divided into four groups：group HS（n=6）：high-fat diet＋single streptozotocin intraperitoneal injection（120 mg/kg）;group H（n=6）：high fat diet＋buffer injection;group S（n=6）：standard chow diet＋single streptozotocin intraperitoneal injection（120 mg/kg）;group C（n=6）：standard chow diet＋buffer injection.The 24 th week was the end point of the experiment,and random glucose,homeostasis model assessment of insulin resistance（HOMA-IR）,mechanical threshold,and IENFD were measured.Results Group HS had significantly higher random glucose and HOMA-IR than other groups（P0.01）,had significantly lower mechanical threshold than other groups（P0.05）,had significantly lower IENFD than groups S and C at 24 th week（P0.05）;group H had significantly higher random glucose and HOMA-IR than group C at 24 th week（P0.01）,had no significant difference in mechanical threshold compared with group S and group C,and had no significant difference in IENFD compared with group HS.Conclusion A mouse model of type 2 diabetes peripheral neuropathy was successfully established,and the IENFD was found to be decreased significantly.

关 键 词：2型糖尿病 糖尿病周围神经病变 小鼠模型 皮下神经纤维密度检测 

分 类 号：R-332[医药卫生] R587.2