缺血性脑卒中患者血浆vWF:Ag和S100B检测的临床意义  

Clinical Application of Detection of Plasma vWF:Ag and S100B in Ischemic Cerebral Apoplexy Disease

作　　者：邓凯[1] 许薇 李燕 

机构地区：[1]西安交通大学第一附属医院检验科,西安710061 [2]咸阳市第一人民医院检验科,陕西咸阳712000

出　　处：《现代检验医学杂志》2017年第5期52-54,58,共4页Journal of Modern Laboratory Medicine

摘　　要：目的探讨血浆钙连接蛋白B(S100B)和血浆血管性血友病因子抗原(vWF:Ag)水平在缺血性脑卒中疾病中的临床应用价值。方法收集85例于2014年1～10月在西安交通大学第一附属医院神经内科住院治疗的缺血性脑卒中患者的血液及临床资料,并从正常人群中随机抽取38例志愿者作为对照组。采用ELISA法检测所有研究对象的血浆S100B水平,使用Sysmex CA-7000全自动血凝仪检测其血浆vWF:Ag水平。采用GELOGIC9型彩色多普勒超声诊断仪分析所有研究对象的颈动脉内径及内中膜厚度。将脑卒中症患者分为完全卒中(CS)组、不完全卒中(NCS)组,与对照组比较,观察血浆S100B和vWF:Ag水平变化与缺血性脑卒中疾病之间的关系。结果血浆vWF:Ag(%)在CS组、NCS组和对照组的水平为176.8±56.3,128.9±37.6,93.7±30.1,血浆S100B水平(μg/L)为2.8±1.3,1.9±0.9,1.3±0.7。在CS组、NCS组和对照组中,各研究对象的颈动脉内径(mm)为(6.27±0.32,6.52±0.57,6.78±0.73,内中膜厚度(mm)为0.88±0.09,0.71±0.07,0.52±0.06。两病例组血浆vWF:Ag和S100B水平与对照组比较均增高,其差异均有统计学意义(F=125.9,89.3,均P<0.01);CS组与NCS组vWF:Ag和S100B比较显著增高,差异均有统计学意义(F=125.9,89.3,P<0.01)。CS组血浆vWF:Ag和S100B水平呈正相关(r=0.836,P<0.01),CS组的血浆vWF:Ag水平分别与颈动脉内径及内中膜厚度具有相关性(r=-0.853,0.923,P<0.01)。CS组的血浆S100B水平分别与颈动脉内径及内中膜厚度具有相关性(r=-0.820,0.833,P<0.01)。结论检测vWF:Ag和S100B水平可预测缺血性脑卒中疾病发生的危险程度,估计病情的发展状况。Objective To explore the clinical application value of S100B and vWF： Ag levels in ischemic cerebral apoplexy disease. Methods The plasma and clinical data from 85 cases of patients with ischemic cerebral apoplexy were collected,extracted 38 cases of volunteer blood plasma from a random sample of the general population and quantitative detected S100B levels by ELISA method, using Sysmex CA 7000 automatic blood coagulation instrument to detect plasma vWF：Ag levels. The carotid artery diameter and intima media thickness of all the participants were detected by GELOGIC9 color doppler ultra- sonic diagnostic instrument. Results The levels of plasma vWF： Ag in the CS group, the NCS （%） group and the control group were 176.8±56.3,128.9±37.6 and 93.7±30.1,which of plasma S100B （μg/L） were 2.8±1.3,1.9±0.9 and 1.3 ±0.7. Stroke patients could be divided into CS group and NCS group, compared with control group, observed the changes and the relationship of ischemic stroke disease for plasma S1OOB and vWF： Ag levels. The levels of plasma vWF.. Ag and called S100B in the two case groups were increased compared with those in controls, the differences had statistical signifi- cance （F= 125.9,89.3, P〈0.01 ）. In CS and NCS group, vWF： Ag and S100B were comparative differences and statistically significant （F= 125.9,89.3 ,P〈0. 01 ）, the levels of the two markers in the CS group were higher than in replication group. In CS group, the plasma levels of vWF .. Ag and S100B were positively correlated （r= 0. 836, P〈0.01）. In CS group, plasma levels of vWF.. Ag were associated with carotid artery diameter and intima media thicknes （r=-0. 853,0. 923, P〈0.01 and r=-0. 783,0. 823,P〈0. 01） ,which of S100Bdid so （r=-0.820,0.833,P〈0.01）.Conelution Detection of the levels of vWF： Ag and S100B can predict the degree of danger of ischemic cerebral apoplexy disease and estimate the development of the disease.

关 键 词：缺血性脑卒中 完全卒中 不完全卒中 血管性血友病因子抗原(vWF:Ag) 血浆钙连接蛋白B (S100B) 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R446.111[医药卫生—临床医学]