江苏地区对虾3种病毒病的流行病学调查及5株IHHNV的编码区基因序列分析  

Prevalence of three viruses in Pacific white leg shrimp in Jiangsu Province and the analysis of coding sequence of five strains of infectious hypodermal and hematopoietic necrosis virus

作　　者：王筱珊 胡智博[1] 费荣梅[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095

出　　处：《水产学报》2017年第10期1623-1630,共8页Journal of Fisheries of China

基　　金：公益性行业(农业)科研专项201103034~~

摘　　要：对虾白斑综合征病毒(WSSV)、桃拉综合征病毒(TSV)、传染性皮下及造血组织坏死病毒(IHHNV)是威胁对虾养殖的重要病毒。为了调查这3种病在江苏境内的流行情况,根据世界动物卫生组织(OIE)推荐的《水生动物疾病诊断手册》的PCR检测法对2015年5月—2016年5月采集的1436尾对虾样品进行3种疾病的流行病学调查。结果显示WSSV阳性率为17.20%,TSV阳性率为0%,IHHNV阳性率为39.48%。对江苏不同地区分离出的5株IHHNV进行基因序列分析,数据显示这5个地区的毒株均属于Ⅰ型感染株,与韩国株的进化关系较为接近。本研究通过调查以上3种病毒病在江苏境内的流行情况,并进行序列分析,发现江苏不同地区的凡纳滨对虾IHHNV感染均为Ⅰ型,研究结果对养殖虾的疾病防控有重要参考价值。White spot syndrome virus（WSSV）, Taura syndrome virus（TSV） and infectious hypodermal and hematopoietic necrosis virus（IHHNV） are the three major pathogenic viruses responsible for the epidemic diseases threatening Pacific white leg shrimp farming industry. We use the PCR detection methods recommended by World Organisation for Animal Health to investigate the epidemiology of these three kinds of diseases in Jiangsu province. 1436 Pacific white leg shrimp samples were detected in the year from 2015 to 2016. Results showed the positive rate of WSSV infection was 17.20%, 0% in TSV infection and 39.48% in IHHNV infection where shrimp samples were collected from Nanjing, Dongtai, Dafeng, Ganyu, and Sheyang. And all five IHHNV strains detected belong to the infectiontype after gene sequence analysis, which were close to the Korean strain. Our investigation aims to clarify the epidemic stiutation of WSSV, TSV and IHHNV in Jiangsu province, identify IHHNV strain type via sequence analysis. The result has a high reference value for diseases prevention and treatment of shrimp farming industry.

关 键 词：WSSV TSV IHHNV IHHNV编码区序列 感染与流行 

分 类 号：S945.1[农业科学—水产养殖]