DNMT3b基因在骨髓瘤RPMI 8226细胞株中的表达及其生物学意义  

Expression of DNMT3b Gene in Myeloma RPMI 8226 Cells and Its Biological Significance

作　　者：王晓宁[1] 姚建娜[1] 王晓娟[1] 孙春红[1] 郭彩利[1] 陈颖[1] 贺鹏程[1] 张梅[1] 

机构地区：[1]西安交通大学第一附属医院血液内科,陕西西安710061

出　　处：《中国实验血液学杂志》2017年第5期1431-1435,共5页Journal of Experimental Hematology

基　　金：陕西省自然科学基础研究计划青年人才项目(2016JQ8057)

摘　　要：目的:研究多发性骨髓瘤细胞株RPMI 8226中DNMT3b基因的表达情况并分析其生物学意义。方法:ELISA方法检测RPMI 8226中DNA甲基转移酶的活性;半定量RT-PCR及实时荧光定量PCR方法检测RPMI 8226细胞中DNMT3b基因mRNA的表达;不同浓度地西他滨干预RPMI8226细胞24 h后观察细胞增殖及DNMT3b基因mRNA表达水平的变化。结果:RPMI8226细胞中DNMT活性升高,DNMT3b基因mRNA表达水平升高。不同浓度地西他滨干预24 h后RPMI8226细胞增殖抑制,发生凋亡,DNMT3b基因mRNA表达水平降低。结论:骨髓瘤RPMI8226细胞中DNMT3b基因表达水平升高,地西他滨可能通过抑制DNMT3b表达从而抑制RPMI8226细胞增殖并诱导其凋亡。因此,DNMT3b有望作为骨髓瘤治疗的新靶点。Objective： To study the expression of DNMT3b gene in myeloma RPMI8226 cells and its biological significance. Methods： The activity of DNA methyltransferase was detected by ELISA,and the expression of DNMT3b in RPMI8226 cells was analyzed by semi-quantitative RT-PCR and real-time fluorescent quantitative PCR. The proliferation and expression of DNMT3b gene in RPMI8226 cells intervened with capecitabine for 24 hours were detected. Results： The activity of DNMT and expression of DNMT3b in RPMI 8226 cells increased. The proliferation of RPMI8226 cells was inhibited,and the apoptosis occurred in RPMI 8226 cells intervened with capecitabine for 24 hours. The expression level of DNMT3b gene was decreased after being intervened with capecitabine for 24 hours. Conclusion： The expression level of DNMT3b in myeloma RPMI 8226 cells increase,and capecitabine can inhibit the proliferation of RPMI 8226 and induce apoptosis by inhibiting the expression of DNMT3b gene. Therefore,DNMT3b is expected to be a new target for myeloma therapy.

关 键 词：多发性骨髓瘤 RPMI8226细胞株 DNMT3b基因 地西他滨 

分 类 号：R733.3[医药卫生—肿瘤]