T淋巴细胞亚群及外周血IL-17、IL-35和IFN-γ在多发性骨髓瘤患者中的水平及其临床意义  

Levels of T-Lymphocyte Subsets, IL-17, IL-35 and IFN-γ in Peripheral Blood and Their Clinical Significance in Patients with Multiple Myeloma

作　　者：徐昭娟 赵冬 李付平 王英芳 肖汇颖 王旭 

机构地区：[1]河北中医学院护理学院,河北石家庄050200 [2]河北中医学院教务处临床教学管理科,河北石家庄050200 [3]河北中医学院河北中医学院附属医院血液科,河北石家庄050011

出　　处：《中国实验血液学杂志》2017年第5期1444-1448,共5页Journal of Experimental Hematology

基　　金：河北省中医药管理局2017年度科研计划项目(2017006)

摘　　要：河北中医学院目的:探讨外周血T淋巴细胞亚群、调节性T细胞(Treg)、白介素IL-17、IL-35和γ-干扰素(IFN-γ)在多发性骨髓瘤(MM)患者中的水平及其临床意义。方法:选取2014年1月-2017年1月医院收治的8~^+例多发性骨髓瘤患者作为MM组,并选取同时期体检健康者30例为对照组。应用流式细胞术检测外周血CD4^+/CD8^+T细胞比例和CD4^+CD25^(high/+)CD127^(low/-)调节性T水平,ELISA法检测血清IL-17、IL-35和IFN-γ表达水平;比较各组不同指标间的差异。结果:与对照组比较,多发性骨髓瘤患者CD4^+T细胞、CD4^+/CD8^+T细胞比例降低,CD8^+T细胞、Treg细胞比例升高;MMⅢ期患者的T淋巴细胞亚群与对照组比较,差异有统计学意义(P<0.05);随着临床分期的提高,Treg细胞呈现增高趋势;MMⅢ期Treg细胞水平明显高于Ⅰ期(P<0.05);血清IL-17水平依次为MMⅢ期>Ⅱ期>Ⅰ期>对照组,血清IL-35和IFN-γ水平依次为对照组>Ⅰ期>Ⅱ期>Ⅲ期(P<0.05);Treg细胞比例依次为进展期>稳定期>对照组(P<0.05);血清IL-17水平进展期>稳定期>对照组,血清IL-35和IFN-γ水平则对照组>稳定期>进展期(P<0.05)。结论:T淋巴细胞亚群、调节性T细胞、IL-17、IL-35和IFN-γ水平异常与多发性骨髓瘤患者疾病进展及预后相关。目的:探讨外周血T淋巴细胞亚群、调节性T细胞(Treg)、白介素IL-17、IL-35和γ-干扰素(IFN-γ)在多发性骨髓瘤(MM)患者中的水平及其临床意义。方法:选取2014年1月-2017年1月医院收治的86例多发性骨髓瘤患者作为MM组,并选取同时期体检健康者30例为对照组。应用流式细胞术检测外周血CD4^+/CD8^+T细胞比例和CD4^+CD25high/^+CD127low/-调节性T水平,ELISA法检测血清IL-17、IL-35和IFN-γ表达水平;比较各组不同指标间的差异。结果:与对照组比较,多发性骨髓瘤患者CD4^+T细胞、CD4^+/CD8^+T细胞比例降低,CD8^+T细胞、Treg细胞比例升高;MMⅢ期患者的T淋巴细胞亚群与对照组比较,差异�Objective： To explore the levels of T-lymphocyte subsets and IL-17,IL-35,IFN-γ in peripheral blood of patients with multiple myeloma（MM） and their clinical significance. Methods： A total of 86 MM patients in our hospital from January 2014 to January 2017 were enrolled in MM group and 30 healthy persons were enrolled in control group,the CD4^＋/CD8 ~＋ T cells ratio,CD4^＋ CD25^high/＋ CD127 ^low/- Treg level in peripheral blood were detected by flow cytometer. The levels of IL-17,IL-35 and IFN-γ in peripheral serum were detected by ELISA,and the differences of detected indicators between different groups were compared. Results： Compared with control group,the proportion of CD4^＋ T cells and CD4^＋/CD8 ~＋ T cells ratio decreased,the proportion of CD8 ~＋ T cells and Treg increased in MM group. The differences of T lymphocyte subsets level between group Ⅲ stage of MM and control group were statistically significant（P〈 0. 05）. With enhancing of clinical stages,Treg level showed a increasing trend,especially in Ⅲ stage（P〈 0. 05）,the serum level of IL-17 as followed in turn： Ⅲ stage Ⅱ stage Ⅰstage control,the serum level of IL-35 and IFN-γ as followed in turn： control Ⅰ stage Ⅱ stage Ⅲ stage（P〈 0. 05）. In terms of disease status,the propurtion of Treg cells as fllowed in turn： disease progression stage stable stage control（P〈 0. 05）,the serum level of IL-17 as followed in turn also： disease progression stage stable stage（P〈 0. 05）,while the serum level of IL-35 and IFN-γ as followed in turn： control disease table stage progression stage（P〈 0. 05）. Conclusion： The abnomal level of T-lymphocyte subsets,Treg,IL-17,IL-35 and IFN-γ are related with progression and prognosis of MM patients.

关 键 词：多发性骨髓瘤 T淋巴细胞 调节性T细胞 白介素-17 白介素-35 IFN-Γ 

分 类 号：R733.3[医药卫生—肿瘤]