长链非编码RNA MUC5B-AS1在人肺腺癌细胞系中的功能研究  

Functional study of LncRNA MUC5B-AS1 in lung adenocarcinoma cell lines

作　　者：王贵露 袁帅[2] 胡泽曜 刘庆云[2] 向颖[2] 吴龙[2] 邬娜[2] 李成英[2] 余祖滨[3] 白莉 杨敬源 李亚斐[2] 

机构地区：[1]贵州医科大学公共卫生学院流行病学教研室,贵阳550000 [2]第三军医大学军事预防医学院军队流行病学教研室,重庆400038 [3]第三军医大学新桥医院:胸外科,重庆400037 [4]全军呼吸内科研究所全军呼吸病研究重点实验室,重庆400037

出　　处：《第三军医大学学报》2017年第21期2072-2077,共6页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(81472190,81672316,81171903);重庆市自然科学基金重点项目(CSTC2015jcy BX0110)~~

摘　　要：目的研究lncRNA MUC5B-AS1基因在肺腺癌中的表达情况及对肺腺癌细胞系生物学行为的影响。方法利用RT-PCR、qRT-PCR检测肺腺癌细胞系中MUC5B-AS1基因的表达情况;构建MUC5B-AS1过表达载体,通过转染构建MUC5B-AS1过表达细胞系;采用CCK-8检测肺腺癌细胞的增殖情况;应用Transwell小室检测MUC5B-AS1过表达对肺腺癌细胞系迁移和侵袭的影响。结果检测了MUC5B-AS1在4株肺腺癌细胞系(A549、SPCA1、H1975和H1299)和1株肺正常上皮细胞系(HBE)中的表达情况,发现MUC5B-AS1在H1299细胞系中表达最低,同时A549细胞系表达最高;通过转染构建MUC5B-AS1过表达H1299、A549细胞系,利用qRT-PCR检测细胞系中MUC5B-AS1基因的表达,与对照组比较,MUC5B-AS1基因在H1299、A549细胞系均升高,且有统计学差异(P<0.05);CCK-8检测细胞增殖,与对照组比较过表达MUC5B-AS1对H1299、A549细胞生长并无影响(P>0.05);应用Transwell小室检测MUC5B-AS1过表达对肺腺癌细胞系迁移和侵袭,过表达MUC5B-AS1促进H1299、A549细胞的迁移和侵袭,差异有统计学意义(P<0.05)。结论 MUC5B-AS1可能在肺癌的迁移和侵袭发挥重要作用,但对肺腺癌细胞的增殖能力没有显著影响。Objective To investigate the expression profiles of lncRNA MUC5B-AS1 in lung adenocarcinoma cell lines and the effect of MUC5B-AS1 on the biological behaviors of the lung adenocarcinoma cells. Methods The expression level of MUC5B-AS1 in 4 lung adenocarcinoma cell lines （H1299, H1975, SPCA1 and A549） and a normal lung epithelial cell line （HBE） was detected by reverse transcription PCR （RT-PCR） and real-time quantitative PCR （qRT-PCR）. LncRNA MUC5B-AS1 was overexpressed by transfection of overexpression vector in H1299 and A549 cells. Cell counting kit-8 （CCK-8） assays were performed to measure the proliferation of lung adenocarcinoma cell lines. Transwell chamber assay was performed to observe the cell invasion and migration ability in the MUC5B-AS1 overexpression H1299 and A549 cells. Results MUC5B-AS1 was expressed in 4 strains of lung adenocarcinoma cell lines and normal lung epithelial cell line. Its expression level was quite lower in the H1299 cells, but higher in the A549 cells than other cell lines. After transfection of overexpression vector in the H1299 and A549 cells, the expression of MUC5B-AS1 was remarkably enhanced （P〈0.05）. CCK-8 assays indicated that MUC5B-AS1 overexpression had no effect on the proliferation in H1299 and A549 cells when compared with non-transfected cells （P〉0.05）. Transwell chamber assay showed the MUC5B-AS1 overexpression promoted the migration and invasion in the H1299 and A549 cells （P〈005）. Conclusion MUC5B-AS1 may play an important roles in the migration and invasion in lung adenocarcinoma cells, but had no obvious effect on proliferation.

关 键 词：肺腺癌 长链非编码RNA MUC5B-AS1 细胞增殖 细胞迁移和侵袭 

分 类 号：R394.2[医药卫生—医学遗传学] R730.23[医药卫生—基础医学]