The distribution of calmodulin and Ca^(2+)-activated calmodulin in cell cycle of mouse erythroleukemia cells  

作　　者：You Jinsong, Li Suwen, Wang Duanshun, Zhang Yun, Suen Daye, and Xue Shaobai Department of Biology, Beijing Normal University, Department of Biology, Hebei Normal University, Shijiazhuang,Hebei, China. 

出　　处：《Cell Research》1990年第1期89-94,共6页细胞研究（英文版）

摘　　要：Cell proliferation is accompanied with changing levels of intracellular calmodulin (CaM) and its activation. Prior data from synchronized cell population could not actually stand for various CaM levels in different phases of cell cycle. Here, based upon quantitative measurement of fluorescence in individual cells, a method was developed to investigate intracellular total CaM and Ca2+-activated CaM contents. Intensity of CaM immunoflurescence gave total CaM level, and Ca2+ -activated CaM was measured by fluorescence intensity of CaM antagonist trifluoperazine (TFP). In mouse erythroleuke-mia (MEL) cells, total CaM level increased from G1 through S to G2 M, reaching a maximum of 2-fold increase, then reduced to half amount after cell division. Meanwhile, Ca2+-activated CaM also in creased through the cell cycle (G1 , S, G2M). Increasing observed in G1 meant that the entry of cells from G1 into S phase may require CaM accumulation, and, equally or even more important, Ca2+-dependent activation of CaM. Ca2+- activated CaM decreased after cell divi-sion. The results suggested that CaM gene expression and Ca2+-modulated CaM activation act synergistically to accomplish the cell cycle progression.

关 键 词：CALMODULIN TRIFLUOPERAZINE cell cycle mous erythroleukemia cells. 

分 类 号：Q26[生物学—细胞生物学]