熟地黄、山茱萸、茯苓单煎液与合煎液中毛蕊花糖苷、没食子酸煎出量的变化  

Decoction Amount Changes of Verbascoside and Gallic Acid in Single Decoction and Combined Decoction of Rehmanniae Radix Preparata,Corni Fructus and Poria

作　　者：杨明明 王慧娜[1] 缪红[1,2] 张万红 赵桂琴 

机构地区：[1]承德医学院中药研究所,河北承德067000 [2]河北省中药研究与开发重点实验室,河北承德067000 [3]承德市中医院中医门诊部,河北承德067000

出　　处：《中国药房》2017年第31期4350-4354,共5页China Pharmacy

基　　金：国家科技支撑计划项目(No.2011BAI07B04);河北省教育厅项目(No.ZD2016061);河北省中医药管理局项目(No.2015034);河北省高校重点学科建设项目(No.冀教高[2013]4号)

摘　　要：目的:考察熟地黄、山茱萸及茯苓单煎液与合煎液中毛蕊花糖苷、没食子酸煎出量的变化,为深入研究合煎液的药效物质基础提供科学依据。方法:分别制备熟地黄、山茱萸、茯苓的单煎液、两两合煎液及三药合煎液,采用高效液相色谱法检测各煎液中熟地黄的有效成分毛蕊花糖苷和山茱萸的有效成分没食子酸的煎出量,并进行比较。结果:与熟地黄单煎液比较,茯苓+熟地黄合煎液中毛蕊花糖苷煎出量降低(P<0.01),山茱萸+熟地黄和三药合煎液中毛蕊花糖苷煎出量升高(P<0.01);与山茱萸单煎液比较,各合煎液中没食子酸煎出量均降低(P<0.01)。结论:山茱萸、茯苓与熟地黄合煎时对熟地黄中毛蕊花糖苷的煎出分别具有一定的促进、抑制作用;熟地黄、茯苓与山茱萸合煎对山茱萸中没食子酸的煎出均有一定的抑制作用。推测毛蕊花糖苷可能是合煎液发挥药效作用的主要成分之一。OBJECTIVE：To investigate the decoction amount changes of verbascoside,gallic acid in single decoction and combined decoction of rehmanniae radix preparata,corni fructus and poria,and provide scientific basis for further study of effective substance basis. METHODS：The single decoction,combined decoction with each 2 medicine and combined decoction with the 3 medicines of rehmanniae radix preparata,corni fructus and poria,were respectively prepared. HPLC was adopted to detect and compare the decoction amount of active component verbascoside in rehmanniae radix preparata and active component gallic acid in corni fructus in each group. RESULTS：Compared with single decoction of rehmanniae radix preparata,the decoction amount of verbascoside was decreased in combined decoction of poria＋rehmanniae radix preparata（P〈0.01）,decoction amount of verbascoside was increased in combined decoction of corni fructus＋rehmanniae radix preparata or combined decoction with the 3 medicines（P0.01）. Compared with single decoction of corni fructus,the decoction amount of gallic acid in each combined decoction was decreased（P〈0.01）. CONCLUSIONS：The combined decoction of rehmanniae radix preparata,corni fructus and poria has certain promotion and inhibition effects on the decoction of verbascoside in rehmanniae radix preparata and inhibition effect on the decoction of gallic acid in corni fructus. It is speculated verbascoside may be one of the main components in combined decoction playing the role of effectiveness.

关 键 词：熟地黄 茯苓 山茱萸 合煎 单煎 毛蕊花糖苷 没食子酸 煎出量 

分 类 号：R283[医药卫生—中药学]