上调PTEN基因表达对卵巢癌细胞凋亡及化疗敏感性的影响  

Influence of up-regulated PTEN gene expression on apoptosis and chemosensitivity of ovarian cancer cells

作　　者：张洋[1] 

机构地区：[1]滕州市中心人民医院妇科,山东省滕州277500

出　　处：《中国医师杂志》2017年第10期1509-1512,共4页Journal of Chinese Physician

基　　金：济宁医学院科研基金资助项目（JY2016KJ0456）

摘　　要：目的研究PTEN基因过表达对人卵巢癌HEY细胞系凋亡和对化疗敏感性的影响。方法将外源性PTEN真核表达质粒转染HEY细胞（PTEN-HEY），以转染空载体（pcDNA-HEY）和未转染细胞（HEY）作为对照组。用蛋白免疫印迹和qRT—PCR检测各组细胞PTEN蛋白和mRNA转录表达情况；流式细胞术评估PTEN过表达对HEY细胞凋亡的影响；裸鼠模型肿瘤形成实验评估体内致瘤性；MTT检测评估PTEN过表达肿瘤细胞对顺铂药物敏感性。结果与pcDNA—HEY组和HEY组相比，转染PTEN基因（PTEN-HEY组）24h后，PTEN蛋白和mRNA表达水平显著增加（P（0．05）。与pcDNA-HEY组和HEY组相比，PTEN—HEY组细胞凋亡率均显著增加（P〈0．05）。将转染细胞接种到裸鼠中，所有裸鼠的接种位点都长出移植瘤，PTEN-HEY组中肿瘤的体积增长显著小于pcDNA—HEY组（P〈0．05），且PTEN—HEY组中肿瘤的重量显著小于pcDNA-HEY组（P〈0．05）。4μg／ml顺铂培养细胞的第4、5、6天时，PTEN-HEY组细胞存活率显著低于HEY组和pcONA—HEY组（P〈0．05）。结论上调PTEN基因促进人卵巢HEY细胞系的凋亡，并增强其对顺铂的化疗敏感性。Objective To explore the influence of up-regulated PTEN gene expression on apoptosis and ehemosensitivity of ovarian cancer cells. Methods HEY cells transfected by exogenous PTEN plasmid （PTEN-HEY） were used as the PTEN-HEY group, and those transfected by empty vectors （pcDNA-HEY） and non transfected ceils （HEY） were used as the control groups. The expressions of PTEN protein and mRNA in each group were detected with Western blot and quantitative real - time polymerase chain reaction （qRT-PCR） , the influence of PTEN overexpression on HEY cells apoptosis was evaluated with flow eytometry, and tumorigenicity in vivo was evaluated with nude mouse model tumor formation test, and cisplatin sensitivity of tumor cells was evaluated with methyl thiazolyl tetrazolium （MTT） detection in condition of PTEN overexpression. Results Compared to those in the pcDNA-HEY group and HEY group, PTEN protein and mRNA expression levels significantly increased after PTEN gene transfected （PTEN-HEY group） in 24 h. Compared to those in the peDNA-HEY group and HEY group, the apoptosis rate of PTEN-HEY group increased significantly. Transfected cells were inoculated into nude mice, and there were transplanted tumors at inoculated sites of all mice, and tumor growth in the PTEN-HEY group was significantly lower than that in the pcDNA-HEY group, and the weight was significantly lower than that in the peDNA-HEY group. At 4th, 5th and 6th day of the cells cultured with 4 p,g/ml cisplatin, cell survival rate in the PTEN-HEY group was significantly lower than those in the HEY and pcDNA-HEY groups. Conclusions Up-regulating PTEN gene expression can promote apoptosis in HEY of human ovarian cell line, and increase the sensitivity to cisplatin chemotherapy.

关 键 词：PTEN磷酸水解酶 卵巢肿瘤/病理学/代谢/药物疗法 细胞凋亡 

分 类 号：R737.31[医药卫生—肿瘤]