GDF11通过激活Smad2/3信号通路抑制小鼠BMSCs骨向分化的体外研究  

GDF11 inhibits osteoblast differentiation of mice-derived BMSCs via activating Smad2/3:a study in vitro

作　　者：朱彪 焦婷[3,2] 赵刚[2] 梅稳 郭希民[4] 郭红延[4,2] 向光大[1,2] ZHU Biao JIAO Ting ZHAO Gang MEI Wen GUO Xi-min GUO Hong-yan XIANG Guang-da(Department of Endocrinology and Metabolism, Wtthan General Hospital of PLA, Hubei Wuhan 430070, China Graduate School of Southern Medical University, Guangdong Guangzhou 510515, China Oncology Division ,Affiliated Hospital of Hebei University, Hebei Baoding 071000, China Stomatology Center, General Hospital of Armed Police Forces ,Beijing 100039, China.)

机构地区：[1]中国人民解放军武汉总医院内分泌与代谢科,湖北武汉430070 [2]南方医科大学研究生院,广东广州510515 [3]河北大学附属医院肿瘤科,河北保定071000 [4]武警总医院口腔医学中心,北京100039

出　　处：《临床口腔医学杂志》2017年第10期579-582,共4页Journal of Clinical Stomatology

基　　金：国家自然科学基金(81570730)

摘　　要：目的:评价生长分化因子11(growth differentiation factor 11,GDF11)对小鼠骨髓间充质干细胞(bonemarrow mesenchymal stem cells,BMSCs)骨向分化的影响。方法:BMSCs随机分为对照组和实验组,成骨诱导培养后,行碱性磷酸酶(ALP)活性检测和Von Kossa染色,实时荧光定量逆转录PCR(qRT-PCR)检测成骨相关基因,Western blot检测Smad的磷酸化水平。结果:GDF11降低ALP活性,减少胞外基质矿化,并通过激活Smad信号通路下调成骨相关基因的表达。结论:GDF11可抑制BMSCs骨向分化。Objective：To investigate the effect of GDF11 on the osteoblast differentiation of BMSCs in vitro.Methods：BMSCs were randomly divided into Vehi group and GDF11 group,after cultured in osteogenic differentiation medium,ALP activity,Von Kossa staining,qRT-PCR and Western blot were tested.Results：GDF11 decreased ALP activity and calcium mineralization,reduced mRNA expression of osteogenic transcription factors by enhancing Samd2/3 signaling pathyway.Conclusion：GDF11 can inhibit BMSCs＇ osteoblast differentiation in vitro.

关 键 词：生长分化因子11(GDF11) 骨髓间充质干细胞 成骨细胞 组织工程骨 

分 类 号：R780.2[医药卫生—口腔医学]