Neu-P11对急性高眼压兔眼内压的影响  

Effects of Neu-P11 on Intraocular pressure in acute high Intraocular pressure Rabbits

作　　者：张瑶[1] 吴鹏[2,3] 尹卫东 贺鹏程[3] 田期先 刘昌汶 刘峰涛 Moshe Laudon[4] 石金凤 

机构地区：[1]常德市第一人民医院检验科,湖南常德415000 [2]衡阳市妇幼保健院重症医学科 [3]南华大学心血管疾病研究所 [4]以色列Neurim Pharmaceuticals Ltd

出　　处：《中南医学科学杂志》2017年第5期471-473,477,共4页Medical Science Journal of Central South China

基　　金：湖南省卫生计生委基金(No C2015-19);南华大学‘蒸湘学者计划’资助(Xiangyang Tang);南华大学"大学生研究性学习和创新性实验计划"(2017XJYZ051;2017XJXZ037)

摘　　要：目的研究吡罗美拉汀(Neu-P11)对急性高眼压兔眼内压的影响。方法采用特伦德伦伯卧位法建立急性高眼压模型兔。建模后将33只急性高眼压新西兰大白兔分为局部给药组和全身给药组,给予生理盐水,褪黑素(Mel)和不同剂量的Neu-P11处理,局部给药通过眼角膜滴注固定体积(10μL)药物进行给药,全身给药通过灌胃固定体积(1 m L)药物进行给药,给药后平位休息2 h,然后再次置于卧位45 min,平位每小时测量一次眼内压,连续监测6 h,重复实验一周。结果新西兰大白兔经Trendelenburg卧位诱导后,眼内压约升高为正常组的1.9倍。局部给药组经NeuP11和Mel治疗后眼内压明显降低(P<0.05),且Neu-P11低浓度治疗组比高浓度组效果更佳。全身给药组Neu-P11和Mel均可明显降低眼内压,且Neu-P11对眼压的影响存在剂量依赖性,Neu-P11浓度越高眼内压下降速度越快。结论 Neu-P11和褪黑素均具有降低急性高眼压兔眼内压的作用。Objective To investigate the effect of piromelatine（ Neu-P11） on intraocular pressure（ IOP） in acute high IOP rabbits. Methods The experiment used the Trendelenburg position to establish acute high IOP rabbit model.33 New Zealand white rabbits with IOP were divided into local treatment group and systemic treatment group.Local or systemic treatment group were treated with saline,melatonin（ Mel） and different concentrations of Neu-P11. The fixed volume（10 μL） was dropped into the cornea in local treatment.The systemic treatment was administered by filling the stomach with drug of fixed volume（ 1 mL）.Rest 2 hours after treatment in the flat supine position,then rabbits were placed in the Trendelenburg position for 45 min.And then,put rabbits in normal position and measured IOP every hour for 6 hours.Repeat the experiment for a week. Results IOP could increase about 1.9 times in the Trendelenburg position of New Zealand white rabbit（ vs Normal group）. Namely acute high IOP model was successfully established. IOP on local treatment with 100/200 μmol/L Neu-P11 or 100 μmol/L Mel significantly declined and the effect of 100 μmol/L Neu-P11 was better than200 μmol/L Neu-P11.Similarly,IOP on local treatment with Neu-P11 and Melatonin could also be reduced,and the treatment of Neu-P11 was dose dependent,the higher the concentration of Neu-P11 was,the faster IOP declined. Conclusions Neu-P11 and Melatonin could significantly reduce IOP of the acute high intraocular pressure rabbit.

关 键 词：Neu-P11 褪黑素 眼内压 

分 类 号：R775[医药卫生—眼科]