高脂饮食诱导C57BL/6J肥胖小鼠骨关节炎模型的建立  

Establishment of osteoarthritis model in C57BL/6J obese mice induced by high fat diet

作　　者：何健宜 姜梦琪 刘旭丹 于晓璐 徐小磊 刘莉 

机构地区：[1]中国医科大学公共卫生学院营养与食品卫生学教研室,沈阳110122

出　　处：《中国组织化学与细胞化学杂志》2017年第5期445-450,共6页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金(81372971)

摘　　要：目的通过高脂饮食诱导C57BL/6J小鼠肥胖来建立骨关节炎模型并观察其病理特征,为肥胖相关骨关节炎研究中动物模型的建立提供实验依据。方法 C57BL/6J雄鼠20只随机分为对照组和高脂组,分别喂饲基础饲料和高脂饲料。每周测体重及摄食量,16周后收集血清,ELISA法检测血清Ⅱ型胶原C端肽水平;取小鼠左下肢膝关节,固定、脱钙、石蜡包埋,切片后行HE及番红O染色并进行修改后的Mankin评分;TUNEL法检测软骨细胞凋亡。结果与对照组相比,高脂组小鼠体重、血清Ⅱ型胶原C端肽水平显著升高,而摄食量方面则无明显差异。HE及番红O染色结果显示,高脂组小鼠的膝关节软骨细胞减少,排列混乱,基质染色不均匀,可出现潮线部分缺失,颜色变浅等;且高脂组的修改后的Mankin评分也显著高于对照组。TUNEL检测结果显示,高脂组小鼠关节软骨凋亡细胞数增多,但与对照组相比差异不显著。结论利用高脂饮食诱导C57BL/6J小鼠肥胖的方法可以建立骨关节炎模型,该模型可用于肥胖相关骨关节炎的研究。Objective Establish an osteoarthritic model in high-fat diet induced obese mice to study obesity-related osteoarthritis （OA）. Methods Twenty C57BL / 6J male mice were randomized into control and high-fat diet groups fed with basic or high-fat diet respectively. Body weight and food intake were measured weekly. Sixteen weeks later serum samples were collected to determine serum collagen typeⅡ C-telopeptide （CTX-Ⅱ） levels by ELISA. Their left knee joints were fixed, decalcified and paraffin embedded for histological examination （HE and Safranin O staining）, modified Mankin scoring as well as apoptosis detection （TUNEL assay）. Results Compared with control mice, the body weight and serum CTX-Ⅱ level significantly increased in obese mice, while food intake showed no difference. HE and Safranin O staining indicated reduced and disorganized articular chondrocytes, discontinuity of tidemark and poor staining in the knee joints of high-fat diet fed mice. The modified Mankin scores in high-fat diet group were significantly higher than that in control group. TUNEL assay showed that compared to control mice, the number of apoptotic cells in articular cartilage increased in the high-fat diet fed mice which however was not statistically significant. Conclusion Osteoarthritic model in obese mouse, which may be suitable for obesity related OA study, can be established through feeding mouse with high-fat diet.

关 键 词：高脂饮食 肥胖 骨关节炎 动物模型 小鼠 

分 类 号：R684.3[医药卫生—骨科学]