AMFR和C-Met在非小细胞肺癌组织中的免疫组织化学表达及其与肿瘤增殖活性的关系  

Immunohistochemical expressions of AMFR and C-Met in non-small cell lung cancer and their relationship with tumor proliferative activity

作　　者：张祖华 李鸣 董利民 江兴堂[3] 

机构地区：[1]萍乡市人民医院,萍乡337000 [2]湘雅萍矿合作医院,萍乡337000 [3]厦门大学附属中山医院,厦门361000

出　　处：《中国组织化学与细胞化学杂志》2017年第5期475-480,共6页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的通过研究非小细胞肺癌组织中自分泌运动因子受体(autocrine motility factor receptor,AMFR)与肝细胞生长因子受体(hepatocyte growth factor receptor,HGF-R/C-Met)的表达,探讨二者与临床病理特征的关系及其与肿瘤细胞增殖活性的关系。方法采用SP法免疫组织化学检测肿瘤组织中AMFR、C-Met和PCNA的表达,并计算增殖细胞核抗原指数(PCNA Index,PI)。结果 AMFR表达的总阳性率为54.1%,其中,低分化组AMFR阳性表达率明显高于中-高分化组;有淋巴结转移组阳性表达率明显高于无淋巴结转移组阳性表达率。C-Met的阳性率为61.3%,其中,低分化肿瘤组织中C-Met阳性表达率明显高于中-高分化组;有淋巴结转移组阳性表达率明显高于无淋巴结转移组阳性表达率;TNM分期I-IIIA期中C-Met阳性表达率为53.7%显著低于IIIB-IV组阳性表达率。此外,AMFR与C-Met的表达率呈显著正相关,AMFR、CMet阳性表达组的PI值明显高于阴性组。结论 AMFR、C-Met两者可能在非小细胞肺癌的发展、转移中起着重要的作用。Objective To study the expression of autocrine motility factor receptor （AMFR） and hepatocyte growth factor receptor （HGF-R/C-Met） in non-small cell lung cancer （NSCLC）. The relationship between the expressions and cancer clinicopathologic features as well as proliferative activity was also explored. Methods The expressions of AMFR, C-Met and proliferating cell nuclear antigen （PCNA） in tumor tissue specimens were examined using S-P immunohistochemistry. The PCNA Index （PI） was calculated. Results The expression of AMFR highly correlated with C-Met. The overall positive rates were 54.1% （AMFR） and 61.3% （C-Met） in all investigated cases, where patients with poorly differentiated tumor cells and lymph node metastasis had significantly higher positivity than those with well differentiated cells and clean lymph nodes. C-Met expression was also found to correlate with TNF classification where its positive rate was 53.7% and 82.8% in I-IIIA and IIIB-IV respectively. The PCNA Indexes were significantly higher in AMFR and/or C-Met positive tumors than those negative ones. Conclusion The expression of AMFR and C-Met had strong correlation with clinicopathological features and tumor cell proliferation which may suggest they play an important role in the progression and metastasis of the cancer.

关 键 词：非小细胞肺癌 自身分泌活动因子受体 肝细胞生长因子受体 细胞增殖 

分 类 号：R73-37[医药卫生—肿瘤]