5-烷基间苯二酚诱导人结直肠癌细胞凋亡的机制探讨  

Mechanism study of 5-alkylresorcinols-induced colon cancer cell apoptosis in vitro

作　　者：杨春[1] 李恒[1] 李海[1] 王宇峰 孟玲 杨银学[1] Chun Yang Heng LI Hai LI Yu-Feng Wang Ling Meng Yin-Xue Yang(Department of Anal-Colorectal Surgery, the General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, Chin School of Clinical Medicine, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, Chin)

机构地区：[1]宁夏医科大学总医院结直肠外科,宁夏回族自治区银川市750004 [2]宁夏医科大学研究生院,宁夏回族自治区银川市750004

出　　处：《世界华人消化杂志》2017年第29期2621-2630,共10页World Chinese Journal of Digestology

基　　金：宁夏回族自治区自然科学基金;No.NZ16136~~

摘　　要：目的探讨5-烷基间苯二酚(5-alkylresorcinols,5ARs)诱导人结直肠癌细胞凋亡及与BCL2、Bax、PARP1和Caspase3表达的影响.方法体外细胞实验应用5AR s直接对HT29、HCT116进行干预诱导.利用倒置相差显微镜观察5ARs对HT29、HCT116干预后细胞形态的变化.CCK8法测定不同浓度5ARs诱导人HT29及HCT116抑制率.Annexin V-F ITC/P I流式细胞术检测5ARs诱导人HT29及HCT116凋亡.Western-blot检测分析不同浓度5ARs诱导HT29及HCT116后凋亡相关蛋白变化(BCL2、Bax、PARP1和Caspase3).结果5A R s可呈浓度依赖性地抑制H T 2 9及HCT116增殖(P<0.05);5ARs可诱导HT29及HCT116凋亡,并可增强Bax、PARP1和Caspase3表达,抑制BCL2表达,并提高Bax/BCL2的比值比例(P<0.05).结论5ARs可诱导HT29及HCT116凋亡,并且可能与激活Bax、PARP1和Caspase3表达及增加Bax/BCL2比值比例相关.AIM To investigate the mechanism of 5-alkylresorcinols（5 ARs）-induced apoptosis by detecting the effect on BCL2,Bax,PARP1 and Caspase3 expression patterns in colon cancer cell lines.METHODS After HT29 and HCT 116 cells were treated with different concentrations of 5 ARs,cell morphological changes were observed by phasecontrast microscopy,cell proliferation and apoptosis phenotypes were evaluated by Cell Counting Kit-8（CCK8） and Annexin V-FITC/PI flow cytometric assays,respectively,and the protein levels of BCL2,Bax,PARP1 and Caspase3 were detected by Western blotting assays.RESULTS CCK8 assays indicated that the proliferation of HT29 and HCT 116 cells treated with different concentrations of 5 ARs was significantly decreased（P 〈0.05）,and treatment with 5 ARs increased the apoptosis of HT29 and HCT 116 cells.5 ARs suppressed BCL2 expression and elevated the levels of Bax,PARP1,Caspase3 and the ratio of BAX to BCL2 in colon cancer cells.CONCLUSION5 ARs can induce the apoptosis of HT29 and HCT 116 cells in vitro,possibly by enhancing BAX,PARP1 and Caspase3 expression and elevating the ratio of BAX to BCL2 in colon cancer cells.

关 键 词：5-烷基间苯二酚 细胞凋亡 结直肠肿瘤 Bax/BCL2比值 

分 类 号：R735.3[医药卫生—肿瘤]