miR-181家族在胶质母细胞瘤中的表达及其临床意义  

Expression of miR-181 family in glioblastoma and its clinical significance

作　　者：王成德[1] 李群[1] 卢江龙[1] 蔡霖[1] 高志超[1] 苏志鹏[1] WANG Chengde LI Qun LU Jianglong et al(Department of Neurosurgery, the First Affiliated Hospital of Wenzhou Medical University, Whenzhou 325000, Chin)

机构地区：[1]温州医科大学附属第一医院神经外科,325000

出　　处：《浙江医学》2017年第18期1518-1523,I0001,共7页Zhejiang Medical Journal

基　　金：浙江省自然科学基金项目(LY16H160053);浙江省医药卫生科技计划项目(2012RCA042);温州市科技计划项目(Y20120008)

摘　　要：目的探讨miR-181家族在人多形性脑胶质母细胞瘤(GBM)中的表达及其与临床病理特征的关系。方法采用茎环实时荧光定量PCR(RT-q PCR)法检测40例胶质母细胞瘤中miR-181a、miR-181b、miR-181c、miR-181d的表达水平,并分析其与胶质母细胞瘤临床病理特征的关系。采用慢病毒转染上调胶质瘤细胞系U251 miR-181a的表达,分析miR-181a对U251克隆形成、细胞增殖和凋亡的影响。结果 miR-181a高表达的GBM患者生存预期明显延长(P<0.05);且miR-181a表达水平越高,GBM瘤周水肿越明显(P<0.05)。O6-甲基鸟嘌呤-DNA甲基转移酶(MGMT)阳性组的GBM miR-181a表达明显高于阴性组(P<0.05)。上调miR-181a的表达后,U251胶质瘤细胞克隆形成数明显少于对照组(P<0.05),且细胞生长抑制在第5天最明显,差异有统计学意义(P<0.01);对细胞凋亡起促进作用(P<0.05)。结论 miR-181a表达水平与GBM患者的临床预后相关。miR-181a表达减少与GBM的发生密切相关。Objective To investigate the expression of miR-181 family in multiform glioblastoma（GBM）and its correlation with clinicopathological characteristics of the tumor. Methods The expression of miR-181a, miR-181b, miR-181c and miR-181d was detected by loop RT-qPCR in tumor tissue specimens of 40 GBM patients. The relationship between miR-181 expression and clinicopathological characteristics was analyzed. The lentiviral vector carrying miR-181a was transfected into U251 ceils, the proliferation clone-forming ability and apoptosis of miR-181a-transfected U251 cells were determined Results The up-regulation of miR-181a expression was correlated with the survival of patients and peritumoral brain edema （P〈0.05）. Expression of miR-181a was significantly higher in patients with O6-methylguanine-DNA methyltransferase（MGMT） positive than those with MGMT negative （P〈0.05）. Cell studies indicated that the over-expression of miR-181a significantly inhibited the proliferation of U251 cells（P〈0.01）, reduced clone-forming ability （P〈0.05）, and increased apoptosis （P〈0.05）. Conclusion The expression of MiR-181a may be associated with the prognosis of GBM, and down-expression of miR-181a may be involved in the development of GBM.

关 键 词：miR-181 胶质母细胞瘤 MGMT 

分 类 号：R739.4[医药卫生—肿瘤]