结直肠癌中miR-21表达与临床病理因素及预后的关系  

Expression of miR-21 in colorectal cancer and its clinicopathologic significance

作　　者：任约翰[1] 叶星照[1] 厉金雷[1] 陈积贤 REN Yuehan YE Xingzhao LI Jinlei et al(Department of Colorectal and Anal Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Chin)

机构地区：[1]温州医科大学附属第一医院结直肠肛门外科,325000 [2]温州医科大学附属第三医院肿瘤外科

出　　处：《浙江医学》2017年第18期1544-1547,共4页Zhejiang Medical Journal

基　　金：浙江省自然基金项目(Y2100917)

摘　　要：目的探讨结直肠癌组织中miR-21表达与临床病理因素及预后的关系。方法取59例结直肠癌组织标本及癌旁正常黏膜组织标本,采用茎环逆转录实时荧光定量PCR方法检测结直肠癌组织及癌旁正常黏膜组织中miR-21的相对表达量,并分析癌组织中miR-21表达与临床病理因素的关系。用Kaplan-meier法计算生存率,采用log-rank检验、Cox比例风险回归模型分析结直肠癌预后影响因素。结果结直肠癌组织中miR-21的相对表达量明显高于其癌旁正常黏膜组织(P<0.05)。结直肠癌组织miR-21表达水平与肿瘤直径、淋巴结转移、临床分期有关(均P<0.05);与性别、远处转移、分化程度、浸润深度和肿瘤位置无关(均P>0.05)。远处转移、分化程度、miR-21表达水平是影响患者预后的独立危险因素(均P<0.05),其RR值及95%CI分别为5.053(1.395~5.787)、3.998(1.395~18.305)、3.837(1.266~11.631)。结论 miR-21与结直肠癌患者预后和疾病进展密切相关。Objective To investigate the expression of miR-21 in colorectal cancer and its association with clinicopathological characteristics and the prognosis of patients. Methods The expression of miR-21 in 59 specimens of colorectal cancer and matched non-tumor adjacent tissue specimens was examined with stem-loop real-time RT-PCR. The relationship between the expression of miR-21 and clinicopathologic features of colorectal carcinoma was analyzed. Kaplan-Meier survival curves, log-rank test and multivariate Cox regression were used to analyze the factors related to prognosis of colorectal cancer. Results The expression level of miR-21 was significantly higher in colorectal cancer tissue than that in the matched normal colorectal mucosa （2.698 ± 1.973 vs. 0.653 ± 0.213, P〈0.05）. The expression of miR-21 was positively correlated with tumor size, lymph node metastasis and clinical stage （P〈0.05）, but not with sex, distant metastasis, cell differentiation, depth of tumor infiltration and location （P 〉0.05）. Kaplan-Meier univariate analysis showed that distant metastasis, cell differentiation, depth of tumor infiltration and miR-21 was the prognostic factor for colorectal cancer （P〈0.05）. The Cox regression analysis showed that distant metastasis, cell differentiation and miR-21 was an independent prognostic risk factor of colorectal cancer（P〈 0.05）, RR value and 95%CI was 5.053 （1.395-5.787）, 3.998 （1.395-18.305） and 3.837 （1.266-11.631）. Conclusion The results indicate that miR-21 serves as a molecular marker for disease progression and prognosis of colorectal cancer.

关 键 词：结直肠癌 MIR-21 预后 COX比例风险回归模型 

分 类 号：R735.34[医药卫生—肿瘤]