高脂血症性急性胰腺炎仓鼠胰腺微循环观察  

Observation of pancreatic microcirculation in hyperlipidemic acute pancreatitis of hamster model

作　　者：王文强[1] 项和平[1] 王伟[1] 李贺[1] 高明[1] 葛魏巍[1] Wang Wenqiang Xiang Heping Wang Wei Li He Gao Ming Ge Weiwei(Department of Emergency Surgery, the Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China)

机构地区：[1]安徽医科大学第二附属医院急诊外科,合肥230601

出　　处：《中华急诊医学杂志》2017年第11期1274-1278,共5页Chinese Journal of Emergency Medicine

基　　金：安徽省自然科学基金（1508085SMH225）

摘　　要：目的建造高脂血症性急性胰腺炎实验动物模型，观察高脂血症对急性胰腺炎的胰腺微循环影响。方法取健康仓鼠30只随机（随机数字法）分为2 组：血脂正常组（20只）、高脂血症组（10只），分别予以普通和高脂饲料喂养，4周后由血脂正常组筛出10只血脂正常且肝胆胰无病变者，随机（随机数字法）均分为正常对照组（C 组）和急性胰腺炎组（AP 组），由高脂血症组筛出5只血甘油三酯升高3倍以上且肝胆胰无病变者，作为高脂血症性急性胰腺炎组（HLAP 组）。按照Schmidt 法制作动物模型, C组仅适当翻动十二指肠与胰腺并进行穿刺操作， HLAP 组和AP 组行微量输液泵匀速、逆行向仓鼠的胆胰管内泵入3.5%牛磺胆酸钠，6 h后于下腔静脉泵入吖啶橙（2 mL/kg），注射完毕后以激光共聚焦显微镜荧光成像系统动态观察活体动物模型胰腺微循环，检测胰腺微循环血管平均直径（microvaseular diameter, MVD）、功能性毛细血管密度（funetional capillary density, FCD）及测量微循环血流速度（microvessel flow velocity, MFV）、白细胞黏附。三组均取胰腺组织制成病理切片，进行胰腺病理评分。结果与AP组、C组比较，HLAP 组胰腺微循环功能指标MVD（μm）、FCD（个/mm^2）、MFV（μm/s）显著降低（MVD：HLAP组2.40±0.26和AP组5.54±0.43、C组7.56±0.42；FCD：HLAP组4.20±0.84和AP组7.56±1.14、C组11.40±1.14；MFV：HLAP组58.80±9.63和AP组131.00±12.94、C组224.40±15.63；均 P〈0.05）,白细胞黏附（个/mm^2）明显增加（HLAP组343.60±13.86和AP组114.00±8.03、C组18.80±2.28,P〈0.05），胰腺病理评分升高（HLAP组10.00±1.59和AP组6.60±1.14、C组1.00±0.71，P〈0.05）。结论高脂血症可加重急性胰腺炎实验动物的微循环系统损伤，促进微循环障碍。Objective To observe the effect of hyperlipidemia on pancreatic microcirculation in an intact hyperlipidemic acute pancreatitis animal moldel. Methods Among 30 healthy hamsters enrolled, 20 were fed normally while, the other 10 were given high fat feed for four weeks. Then 10 animals given normal feed with normal serum lipids and no ultrasonic changes in the organs including liver, biliary tract and pancreas, were randomly （random number） selected and divided into normal control group （C group） and acute pancreatitis group （AP group ） averagely. Five animals given high fat feed with 3 times serum normal triglyeeride level and no ultrasonic changes in the organs including liver, biliary tract and pancreas, were randomly selected as hyperlipidemic acute pancreatitis group （HLAP）. The animals in C group were undergone fake surgery. While the animals in other two groups were carried out surgery based on Schmidt method and were injected 3.5% taurocholic acid sodium into pancreatic duct with a infusion pump. Acridine orange （2 mL/kg） was injected in the inferior vena cava 6 hours later, then laser confocal microscope fluorescence imaging system were utilized to dynamically observe pancreatic microcirculation in living animal models and the variables including average microvascular diameter （ MVD）, functional capillary density （FCD）, microvessel flow velocity （MFV） and leukocyte adhesion were recorded. Pancreatic tissue were collected and cut slices for pathological grading. Result When compared with AP group and C group, pancreas microcirculation function index MVD （ μm）, FCD （ piece/mm^2）, MFV （μm/s） in the HLAP group decreased, while leukocyte adhesion （piece/mm^2） increased significantly [ MVD： HEAP group 2. 40 ± 0. 26 vs. AP group 5.54 ＋ 0. 43, C group 7. 56 ±0. 42 （ P 〈0. 05 ） ; FCD ： HLAP group 4. 20 ±0. 84 vs. AP group 7.56 ± 1.14, C group 11.40 ± 1.14 （ P 〈 0. 05 ） ; MFV ： HLAP group 58. 80 ± 9. 63 vs. AP group 131.00 ± 12. 94, C group

关 键 词：胰腺炎 高脂血症 仓鼠 动物模型 胰腺微循环 

分 类 号：R576[医药卫生—消化系统] R589.2[医药卫生—内科学]