水黄连抑菌物质提取工艺及其抑菌效果的研究  

Optimizing Extraction Conditions of Antifungal Substance from Thalictrum smithii B. by Response Surface Methodology and Its Inhibitory Effect

作　　者：陈楚英 万春鹏[1,2] 付永琦 甘武[1,2] 彭旋 陈金印[1,2] 

机构地区：[1]江西省果蔬采后处理关键技术与质量安全协同创新中心,江西南昌330045 [2]江西省果蔬保鲜与无损检测重点实验室,江西农业大学,江西南昌330045

出　　处：《热带作物学报》2017年第10期1932-1939,共8页Chinese Journal of Tropical Crops

基　　金：江西省现代农业柑橘产业技术体系(2016);江西省青年科学基金项目(No.20171BAB214031);江西省教育厅科技落地计划项目(111);江西省"赣鄱英才555工程"(2012)

摘　　要：以水黄连提取物对意大利青霉菌的抑菌圈直径为考察指标,在单因素试验基础上,采用4因素3水平的响应面优化水黄连中抑菌物质的提取条件,并将最优工艺所得提取物用于柑橘活体抑菌试验。结果表明:影响水黄连抑菌效果的主次因素为:提取时间>乙醇体积分数>液料比>温度。超声波辅助提取的最佳工艺条件为:乙醇体积分数65%,时间82 min,液料比23 mL/g,温度75℃,获得的抑菌圈直径为(52.33±0.41)mm,与理论最优值52.20 mm接近;水黄连提取物对由意大利青霉菌引发的柑橘青霉病有显著的防治作用,EC_(50)为59.53 mg/mL。Penicillium italicum was used as the tested strain. According to the single-factor experiments, a fourvariable, three-level Box-Benhnken Design（ BBD） matrix was applied to optimize the extraction conditions of antifungal substance from Thalictrum smithii B. by a response surface methodology. The control efficacy of the TSB extract was tested in vivo against blue mold in citrus fruit. The statistical analysis showed that the factors influencing the extracting antifungal substance were in the order： ultrasound extraction timeethanol concentrationliquid-to-solid ratio temperature. The optimum extraction conditions were as following： ethanol concentration of65%, extraction time of 82 min, liquid-to-solid ratio of 23 mL/g and temperature of 75 ℃. Under the above extraction conditions, the average value of inhibitory zone diameter was（ 52.33 ±0.41）mm, well matched with the predicted value of 52.20 mm. The TSB extract had significant efficacy in controlling blue mold in citrus fruit and the EC_（50） value was 59.53 mg/mL in vivo test.

关 键 词：水黄连 超声波提取 响应面法 意大利青霉菌 抑菌效果 

分 类 号：R284.2[医药卫生—中药学]