星形胶质细胞调控少突胶质前体细胞增殖机制研究  

Study on Mechanism of Astrocytes Regulation on Proliferation of Oligodendrocyte Precursor Cells

作　　者：刘兆雨 王尚[1] 徐丹[2] 高晓燕 姜露 唐勇[1] 彭彦[1] 

机构地区：[1]重庆医科大学基础医学院,重庆400016 [2]重庆医科大学附属第一医院神经外科,重庆400016

出　　处：《基因组学与应用生物学》2017年第10期4030-4035,共6页Genomics and Applied Biology

基　　金：国家自然科学基金(81671259;81470057;81501101);重庆市"百名学术学科领军人才"培养计划共同资助

摘　　要：为了探讨星形胶质细胞(AST)与少突胶质前体细胞(OPC)在共培养环境中鞘氨醇激酶1(SPHK1)对少突胶质前体细胞增殖的影响及作用机制。试验采用新生(P0~P3)SD大鼠,培养AST与OPC并建立共培养体系,分为AST与OPC共培养组、SPHK1拮抗剂组。流式检测细胞周期;RT-PCR验证转录组测序结果 SPHK1差异基因表达的准确性及SPHK1抑制后下游p21基因表达;Western blotting检测p21蛋白水平的变化;Ed U检测SPHK1拮抗后OPC增殖能力。试验证明RT-PCR与转录组测序结果一致,SPHK1拮抗后p21蛋白与基因表达均升高,OPC增殖能力下降。因此,我们认为AST主要上调SPHK1表达诱导OPC增殖。The aim of research was to explore the effect and mechanism of SPHK1 on the proliferation of oligodendrocyte progenitor cells（OPCs） in a co-culture of astrocytes（ASTs） and OPCs. Neonatal Sprague-Dawley rats（P0-P3） were used to establish AST-OPC co-culture model, and the rats were divided into two groups as experimental group and SPHK1 blocker group. Cell cycle was tested by flow cytometry. The accuracy of different gene expression in sequencing was verified by RT-PCR. After SPHK1 blocking, p21 expression was verified by RT-PCR and its protein level was assessed by Western blotting. The proliferation of OPC was investigated by Ed U.The results showed that RT-PCR was consistent with the sequencing results of the transcription group, the p21 protein and gene expression were increased, and the proliferation ability of OPC decreased after SPHK1 inhibited. Therefore,we considered that AST might up-regulate the expression of SPHK1 to induce OPC proliferation.

关 键 词：星形胶质细胞 少突胶质前体细胞 SPHK1 P21 

分 类 号：R741[医药卫生—神经病学与精神病学]