山羊CCRL2基因的分子克隆和表达分析  

Molecular cloning and expression analysis of CCRL2 gene in goat(Capra hirus)

作　　者：廖红海 林亚秋[1] 朱江江[1,2] 李倩 林森[1,2] 张小玉 李想[1] 王永 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]青藏高原动物遗传资源保护与利用四川省重点实验室,四川成都610041

出　　处：《中国兽医学报》2017年第11期2175-2180,共6页Chinese Journal of Veterinary Science

基　　金：四川省"十二五"畜禽育种攻关项目(2011NZ0099-36);四川省科技创新产业链示范工程重大项目(2014NZ0003);四川省教育厅项目(15ZA0385)

摘　　要：本研究旨在克隆山羊趋化因子(C-C基序)受体2(chemokine(C C motif)receptor-like 2,CCRL2)基因序列,并研究其组织和时序表达谱。以简州大耳羊为试验材料,应用RT-PCR方法克隆CCRL2基因并进行生物信息学分析,qPCR方法构建该基因在山羊不同组织表达谱和不同月龄背最长肌、股二头肌和臂三头肌中的表达水平,并将基因表达与IMF含量进行相关分析。结果显示:克隆得到山羊CCRL2基因(GenBank登录号:KT165378)长度为1 143bp,编码区长度为1 047bp;qPCR分析表明,CCRL2mRNA在24月龄山羊心、肝、脾、肺、肾、脂肪、背最长肌、股二头肌和臂三头肌中均有不同程度的表达,其中脾表达量最高(P<0.01);时序表达中,CCRL2基因在股二头肌和臂三头肌中的表达量均在8~10月龄极显著高于1~3和24月龄(P<0.01),在背最长肌中则为24月龄极显著高于1~3和8~10月龄(P<0.01);相关分析显示,股二头肌中CCRL2基因mRNA表达量与IMF含量呈极显著负相关(P<0.01)。CCRL2基因可能参与山羊IMF沉积作用。The aim of this study was to clone the goat CCRL2 gene and characterize their tissues and sequential expression profiles. The Jianzhou big-eared goat（Capra hirus） was used in the study. The CCRL2 gene was clone with RT-PCR method,and analyzed by using bioinformatic method. The expression of CCRL2 gene of goat was detected by quantitative RT-PCR in various tissues and in longissimus dorsi,biceps femoris muscle and triceps brachii muscle at different stages. The results showed that the CCRL2 gene （GenBank accession No. ：KT165378） was a length of 1143 bp and coding sequence （CDS） of 1 047 bp. The quantitative RT-PCR indicated that CCRL2 mRNA was widely expressed in various tissues, including heart, liver, spleen, lung, kidney, adipose, longissimus dorsi muscle, biceps femoris muscle and triceps brachii muscle, being the highest in lung （P〈0.01）. Expression of CCRL2 gene in biceps femoris muscle and triceps hrachii muscle in 8-10 month was greater （P〈0.01） than that in 1-3 and 24 month. Expression of CCRL2 gene in longissimus dorsi in 24 month were greater （P〈0.01） than that in 1-3 and 8-10 month. Correlation analysis demonstrated that the expression of CCRL2 in the biceps femoris was extremely negatively related to IMF content （P〈0.01）. In conclusion,the CCRL2 may play important role in regulaing IMF deposition in goat.

关 键 词：山羊 CCRL2基因 克隆 表达谱 肌内脂肪 

分 类 号：S852.2[农业科学—基础兽医学]