卵巢癌肿瘤间质中癌相关成纤维细胞全基因表达谱分析  

DNA microarray screening for ovarian cancer associated fibroblasts

作　　者：侯靓思 叶小琳 令狐华[1] 

机构地区：[1]重庆医科大学附属第一医院妇产科,重庆400016

出　　处：《重庆医科大学学报》2017年第11期1385-1389,共5页Journal of Chongqing Medical University

基　　金：国家自然科学基金面上资助项目(编号:81572562)

摘　　要：目的:寻找上皮性卵巢癌(epithelial ovarian cancer,EOC)癌相关成纤维细胞(cancer associated fibroblasts,CAFs)和正常成纤维细胞(normal fibroblasts,NFs)中差异表达的m RNA并进行分析,为进一步阐明肿瘤间质中癌相关成纤维细胞影响卵巢癌细胞恶性行为的机制提供依据。方法:分别提取3例上皮性卵巢癌的CAFs及3例正常卵巢组织的NFs,抽提总RNA,进行全基因表达谱分析。对差异基因进行基因本体论(Gene Ontology,GO)富集及信号通路联合分析,推定相关差异基因生物学功能,并对MDM2、NR3C1等6个上调的差异基因进行荧光定量PCR验证。结果:对芯片结果的分析显示差异基因共1 636个。q RTPCR对MDM2、NR3C1等6个差异基因的验证结果与芯片结果相符,生物信息学分析显示上述差异基因与癌相关通路、肌动蛋白细胞骨架重塑通路等有关,提示可能参与了对卵巢癌细胞恶性行为的促进作用。结论:EOC肿瘤间质中的成纤维细胞存在调控癌细胞的差异基因,分析这些差异基因为进一步研究卵巢癌成纤维细胞调控癌细胞生物学行为的分子机制提供了基本依据。Objective： To seek the differential genes of cancer associated fibroblasts （CAFs） from normal fibroblasts （NFs） that might function in regulating the malignancy of cancer cells of EOC. Methods： CAFs and NFs were isolated from fresh ovarian tissue of pa- tients of epithelial ovarian cancer（EOC）（n=3） and with benign diseases（n=3）. Total RNAs were extracted for detecting the differen- tially expressed genes between CAFs and NFs through DNA microarray. Gene Ontology and KEGG pathway analysis were conducted using DAVID to analyze the biological function of the deferentially expressed genes, qRT-PCR was applied to verify the reliability of DNA microarray result. Results：Totally 1 636 genes changed were identified in CAFs. qRT-PCR analysis of 6 genes validated the array results. Bioinformatics analysis indicated the likely involvement of these differential genes in carcinogenesis and regulation of actin cytoskeleton, which might promote the malignant behavior of EOC. Conclusion：Differentially expressed genes found in CAFs of EOC provide a basis for further studying the promotive role of CAFs for EOC.

关 键 词：上皮性卵巢癌 癌相关成纤维细胞 全基因表达谱 芯片 

分 类 号：R737.31[医药卫生—肿瘤]