金纳米簇荧光猝灭型探针高灵敏检测精胺  

Highly Sensitive Detection of Spermine Based on Fluorescence Quenching of Gold Nanoclusters

作　　者：安晓刚[1] 杜捷[1,2] 齐伟男 刘璐 李小燕 甘海玲[1] 卢小泉 

机构地区：[1]西北师范大学生命科学学院,兰州730070 [2]甘肃省生物电化学与环境分析重点实验室,兰州730070

出　　处：《分析化学》2017年第8期1209-1214,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21165015;21565020)资助~~

摘　　要：以紫脲酸铵(Murexide,MU)为还原剂及保护剂,采用水热合成法,合成了紫脲酸铵保护的荧光金纳米簇(MU-Au NCs),合成方法简单、快速。基于精胺对MU-Au NCs的荧光猝灭现象,建立了快速、超灵敏检测精胺的"Turn off"型荧光分析方法,在优化的条件下,本方法检测精胺的线性范围为0.003~300μmol/L,检测限为1 nmol/L(S/N=3)。本方法为构建精胺生物传感器及实际样品检测提供了理论基础和参考。A new type of fluorescent gold nanoclusters （ MU-Au NCs） was prepared by hydrothermal synthesis method using ammonium benzoate murexide （MU） as reducing agent and protecting agent. The synthesis method was simple and rapid. Based on the fluorescence quenching ability of spermine, a ＂ turn off＂ type fluorescence analysis method was established for rapid and ultra sensitive detection of spermine. The linear range for detection of spermine was 0.003 - 300μmol/L and the detection limit was 1 nmol/L （ S/N =3）. The established analytical method of spermine provided theoretical basis and reference for construction of spermine biosensor and actual sample detection.

关 键 词：紫脲酸铵 金纳米簇 荧光猝灭 精胺 生物胺 

分 类 号：O657.3[理学—分析化学] TS207.3[理学—化学]