高效液相-激光诱导荧光法测定克拉维酸钾中的黄曲霉毒素  

Determination of Aflatoxins in Potassium Clavulanate by HPLC-LIF

作　　者：孙卓鑫 马海艳[1] 任晓蕾 李梦娇[1] 蒋晔[1] 

机构地区：[1]河北医科大学药学院,河北石家庄050017

出　　处：《中国医药工业杂志》2017年第11期1634-1637,共4页Chinese Journal of Pharmaceuticals

基　　金：河北省省级大学生创业创新训练项目(USIP2016028)

摘　　要：建立了高效液相-激光诱导荧光法检测克拉维酸钾中的黄曲霉毒素B_1、B_2、G_1和G_2。采用Luna C_(18)柱,流动相为甲醇∶乙腈∶水(27∶27∶46),采用0.05%碘溶液柱后衍生,激光器激发波长375 nm,发射波长450 nm。4种黄曲霉毒素(B_1、B_2、G_1和G_2)分离完全,检测限分别为0.08、0.024、0.08和0.024 ng/ml,且分别在0.2~10、0.06~3、0.2~10和0.06~3 ng/ml范围内线性关系良好。黄曲霉毒素B_1、B_2、G_1和G_2的回收率为83.3%~105.0%,RSD为1.2%~3.6%。An HPLC-LIF method was established for the determination of aflatoxin B1, B2, G2 and G2 in potassium clavulanate. A Luna C18 column was used, with the mobile phase of methanol ： acetonitrile：water （27： 27 ： 46）, with 0.05 % iodine solution as the post-column derivatization agent, at the excitation wavelength of 375 nm and emission wavelength of 450 nm. Four aflatoxins （B~, BE, G1 and G2） were well separated. The limits of detection （LODs） were 0.08, 0.024, 0.08 and 0.024 ng/ml for B~, B2, GI and G2, respectively. Their calibration curves were linear in the ranges of 0.2--10, 0.06-3, 0.2--10 and 0.06---3 ng/ml, respectively. Their average recoveries were 83.3 %--105.0%, with RSDs of 1.2 %--3.6 %. The established method improves the detection ability of aflatoxin and it is easy to operate. It provides a more sensitive and reliable method for the drug control.

关 键 词：克拉维酸钾 黄曲霉毒素 高效液相色谱 激光诱导荧光检测器 含量测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]