云南省人类免疫缺陷病毒-1流行株亚型分布及基因变异研究  

Genotype distribution and genetic variation analysis on human immunodeficiency virus-1 epidemic strains in Yunnan Province

作　　者：李健健 张米 董兴齐 李惠琴 雷素云 谢祺 刘家法 

机构地区：[1]云南省传染病专科医院艾滋病关爱中心,昆明650301

出　　处：《中华传染病杂志》2017年第9期546-550,共5页Chinese Journal of Infectious Diseases

基　　金：云南省医疗卫生单位内设研究机构项目（2017NS124）;云南省科技厅应用基础研究计划自筹项目（2013FZ217）

摘　　要：目的研究云南省目前HIV-1流行株的亚型分布及不同亚型基因序列在不同地区的氨基酸变异特点。方法收集2012年至2015年云南省14个地市800例HIV感染者或艾滋病患者的血样及流行病学调查信息，采用反转录-PCR的方法扩增HIV-1的5’半分子基因约4．5kb，序列校对后用Genotyping、MEGA6．06和BLAST工具软件确定亚型，按照采样地点和亚型对序列进行分组并利用Entropy软件分析不同组别的氨基酸序列差异，分析云南省HIV-1亚型的地区分布特征和基因变异特点。结果扩增后800例样本共获得12个地市的466条半分子序列，分型后各种亚型所占比例分别为CRF08-BC（58．3％）、CRF01-AE（19．3％）、CRF07-BC（11．6％）、未知重组（7．1％）、B（B^＋）亚型（1．7％）和C亚型（1．3％）。HIV-1在云南省存在地域分布特征，德宏州、西双版纳和文山州主要以CRF01-AE为主，而临沧、红河州、普洱CRF08-BC占绝对优势（〉70％），其余地州则以CRF08-BC为主，但昆明、玉溪和大理CRF07-BC均〉20％。云南省东部地区和西部地区在3种亚型CRF08-BC、CRF01-AE和CRF07-BC上氨基酸序列存在差异，分别存在17、14、18个氨基酸组成有统计学差异的位点（均P〈0．05）。结论HIV-1在云南省范围内的遗传变异过程中，东、西部毒株氨基酸突变位点存在差异，该差异代表了同种亚型在不同的地域分布下其遗传背景差异和免疫选择的结果，应密切监测其流行趋势变化。Objective To investigate the subtypes distribution of human immunodeficiency virus （HIV） 1 epidemic strains and the characteristics of amino acid variation in different areas of Yunnan Province. Methods Ibtally 800 HIV/AIDS plasma specimens and epidemiological information were collected between 2012 and 2015 from 14 areas of Yunnan. Viral RNA was extracted and amplified using RT-polymerase chain reaction （PCR）. 4.5 kb 5＇ halves fragments were obtained and directly sequenced. Subtypes of strains were identified by Genotyping, MEGA 6.06 and BLAST. Grouping was analyzed by location and subtype. Entropy software was used to analyze the difference of amino acid sequences between different groups according to the sampling location and subtypes to analyze the regional distribution and genetic variation of HIV1 subtypes in Yunnan Province. Restdts Of the 800 plasma specimens, a total of 446 genomic sequences from 12 areas were successfully amplified and sequenced. After genotypes were identified, the subtypes of HIV 1 strains prevalent in Yunnan were CRF08 _ BC （ 58. 3 % ）, CRF01 _ AE （ 19. 3 % ）, CRF07 _ BC （ 11. 6 % ）, unknown recombinant forms （7.1 % ）, B（B＇） （1.7 % ） and C （1.3 %）. The geographical distribution in Yunnan was analyzed. The CRF01_ AE predominated in Dehong, Xishuangbanna and Wenshan. The CRF08_BC predominated in Lincang, Honghe and Puer （more than 70.0 %） and CRF08_BC was prevalent in the other areas. But CRF07_BC in Kunming,Yuxi and Dali accounted for more than 20 %. The constitutions of amino acid of three majors CRF08_BC, CRF01 _AE and CRF07_BC were different on 17, 14 and 18 amino acid sites with statistical differences in the eastern and western regions of Yunnan Province （ P〈 0. 05 ）. Conclusions HIV-1 strains transmit and vary genetically in the province widely. The amino acid mutation sites of eastern and western strains are different. This difference represents that the same subtype strains in different geographical distribution vary on differe

关 键 词：HIV-1 亚型 基因变异 

分 类 号：R512.91[医药卫生—内科学]