胡桃楸活性成分时空动态及抗肿瘤活性初步研究  

Temporal and Spatial Variations of Active Ingredients in Juglans mandshurica Maxim and Their Anti-tumor Activity

作　　者：罗猛 乔琪 牟璠松 阮鑫 杨璇 邢文淼 杨雨春[2] 祁永会 段喜华 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040 [2]吉林省林业科学研究院,长春130033 [3]黑龙江省林业科学研究所,哈尔滨150081

出　　处：《植物研究》2017年第6期947-952,共6页Bulletin of Botanical Research

基　　金：国家重点研发计划项目(2017YFD0600605);黑龙江省博士后科研启动金(LBH-Q13003);国家自然科学基金(21403032)~~

摘　　要：采用超声波辅助提取方法,以胡桃楸为原料,在单因素实验的基础上研究一年内不同月份胡桃楸不同部位总黄酮、总酚的含量变化,同时对胡桃楸提取物体外抗肿瘤活性进行初步评价。结果显示,乙醇浓度60%,超声时间40 min,料液比1∶20,提取温度30℃为最佳提取参数。在此条件下,不同采摘时期胡桃楸不同部位的总黄酮、总酚含量存在显著差异。5~7月份叶和小枝的总黄酮及总酚含量明显高于8~10月份,其中7月份叶的总黄酮总酚含量最高,分别为12.56±0.33和76.98±3.73 mg·g^(-1),然而茎皮总黄酮、总酚含量随月份增加呈持续下降趋势。体外抗肿瘤研究表明胡桃楸叶提取物对人肝癌细胞SMMC-7721、人乳腺癌细胞MCF-7和人口腔表皮样癌细胞KB的增殖抑制作用,其中7月份胡桃楸叶提取物对人乳腺癌MCF-7细胞增殖抑制作用更明显,其IC_(50)值为0.24 mg·mL^(-1)。本研究为胡桃楸资源的合理开发及利用提供一定的理论基础。dynamic c We used uhrasonic-assisted extractioncombined with single factor experiment to determine the hanges of total phenolic and total flavonoid content from different parts of Juglans mandshurica Maxim, and measurethe inhibitive effect of extract from J. mandshurica on tumors. The optimum extraction conditions were ethanol extract fraction of 60%, extract time of 40 rain, solid-liquid ratio of 1：20 and extraction temeperature of 30℃. According to the optimized extraction condition parameters, total flavonoid content, total phenolic content in J. mandshurica were significantly different during growth. Total flavonoid and phenolic contentextracted from leaves and sprig from May to Julyare higher than that from August to October. Total flavonoid and phenolic content in J. mandshurica leaves from July were the highest（ 12.56 ± 0.33 and 76.98 ± 3.73 mg ·g^-1）. However, total flavonoid and phenolic content in the bark were decreased with the growth of J. mandshurica. In vitro anti-tumor studies show extracts from J. mandshurica leaves can inhibit the growth of SMMC-7721, MCF-7 and KB cells. Extracts from the leaves of J. mandshurica in July significantly can inhibit the growth of MCF-7 human breast cancer cells, and the ICs0 is 0.24 mg · mL^-1

关 键 词：胡桃楸 总黄酮 总酚 季节动态 抗肿瘤 

分 类 号：S792.132[农业科学—林木遗传育种]