动物性食品中替米考星酶联免疫检测方法的研究  

Detecting Tilmicosin in Animal Product Using Enzyme Linked Immunosorbent Assay

作　　者：万宇平 韩深[3] 吴小胜 刘萤[3] 孔祥雅 桑旭升 邬良贤 彭鸽 

机构地区：[1]北京勤邦生物技术有限公司,北京102206 [2]北京市食品安全免疫快速检测工程技术研究中心,北京102206 [3]北京出入境检验检疫局检验检疫技术中心,北京100026 [4]福建省龙岩市农产品质量安全检验检测中心,福建龙岩364000

出　　处：《食品研究与开发》2017年第22期158-163,共6页Food Research and Development

基　　金：北京市科技计划课题(Z151100002115059)

摘　　要：通过对替米考星分子结构进行改造,制备替米考星半抗原及人工抗原,通过免疫动物得到替米考星的单克隆抗体。该抗体灵敏度为0.5μg/L,对泰乐菌素的交叉反应率小于1%。基于该抗体建立了替米考星间接竞争酶联免疫检测方法检测动物组织、肝脏、蜂蜜、鸡蛋、牛奶的检测限分别为0.5、10、1、5、5μg/kg,添加水平回收率为70%~110%,变异系数小于15%。Artificial hapten and immunogens were synthesized, after which monoclonal antibodies against tilmicosin was raised. The sensitivity of the antibody was 0.5 μg/L, and it cross-reaction rate of tylosin was less than 1 %. An indirect competitive enzyme immunoassay based on the antibody for the quantitative analysis of tilmicosin was described. The results showed that the limit of detection was 0.5 μ g/kg in animal tissue, 10μ g/kg in liver, 1μg/kg in honey and 5 μ g/kg in eggs,and milk. The average recovery rate of 70 %-110 % , and the coefficients of variation of less than 15 %.

关 键 词：替米考星 单克隆抗体 酶联免疫检测 

分 类 号：TS207.3[轻工技术与工程—食品科学]