一株新的多菌灵降解菌的筛选、鉴定及其降解特性  

The Isolation,Identification and Degradation Character of a Novel Carbendazim-degrading Bacterium

作　　者：李杰[1] 王亦然 年浩瀚 陈梦玲 鲁瑶 王光利[1] 

机构地区：[1]淮北师范大学生命科学学院,安徽淮北235000

出　　处：《淮北师范大学学报（自然科学版）》2017年第3期37-42,共6页Journal of Huaibei Normal University：Natural Sciences

摘　　要：从长期受多菌灵污染的土壤中,通过培养试验和紫外分光光度计法,筛选出一株能较好降解多菌灵的细菌,并将其命名为C4.根据菌落形态、生理生化特性及16S rDNA序列的同源性分析等,初步鉴定为细杆菌属.多菌灵降解菌C4能够适应较广的温度范围,且在30℃生长最好,降解效果最优.当初始p H为7.0,且通气量大的时候,该菌株生长最好,降解率最高.在一定的NaCl浓度范围内,降解菌C4都能生长,且当盐浓度为35 g·L^(-1)时,降解率最高,结合实验所得数据,本次实验选择10 g·L^(-1).在最优降解条件下,降解菌C4在5~6 d内,几乎可以完全降解100 mg·L^(-1)的多菌灵,平均降解能力为18.5 mg·L^(-1)·d^(-1).The aim was to isolation a novel carbendazim-degrading bacterium and study the degradation char-acter. A carbendazim-degrading bacterium was isolated by continuous enrichment,screened from long carben-dazim-treated soils,then it was identified according to their morphological observation,physiological biochem-ical test,comparison sequences of 16 S rDNA and phylogenetic analysis. The degrading rate was detected byUV spectrophotometer. The result shows that the optimum growth temperature on carbendazim is 25~30 ℃,the optimum p H is 7.0. The C4 can utilize carbendazim as the sole carbon or nitrogen source and after fiveto six days 100 mg·L^（-1） of carbendazim could almost be degraded completely,the average degradation rate at18.5 mg·L^（-1）·d^（-1）.

关 键 词：多菌灵 筛选 鉴定 生物降解 

分 类 号：X173[环境科学与工程—环境科学]