高海拔来源芽胞杆菌根腐生防菌的筛选与鉴定  

Screening Bacillus Strains from High-altitude Regions in Search for Identifiable Biological Agents to Control Root Rot Disease in Plants

作　　者：郑梅霞[1] 朱育菁[1] 刘波[1] 陈峥[1] 黄素芳[1] 刘欣[1] 

机构地区：[1]福建省农业科学院农业生物资源研究所,福建福州350003

出　　处：《福建农业学报》2017年第9期996-1000,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划项目--省属公益类科研院所基本科研专项(2015R1018-2);福建省农业科学院科技创新项目(2015CX-7)

摘　　要：本研究从82株高海拔来源菌株中筛选对根腐病具有较强抑制作用的拮抗生防菌。利用平板对峙法初步筛选根腐生防菌,利用牛津杯法测定抑菌活性,筛选获得FJAT-19708和FJAT-20265共2株拮抗根腐的菌株,经16SrRNA基因序列分析均鉴定为Bacillus subtilis枯草芽胞杆菌,有望用于控制植物根腐病。In search of effective biocontrol species with antagonistic activities against root rot disease in plants,82 strains of Bacillus bacteria from the high-altitude regions in China were screened.The initial screening was performed on PDA agar plates,and an Oxford cup method was applied to determine the possibly existing antibacterial activities.In the end,FJAT-19708 and FJAT-20265 showed the strongest activities against the root rot.And,their 16 SrRNA gene sequences were found homologous to that of Bacillus subtilism.

关 键 词：生防菌 枯草芽胞杆菌 根腐 

分 类 号：Q939[生物学—微生物学]