不同样本和方法对测定大鼠血液生化指标的影响  

Effects of different samples and methods on the determination of blood biochemical indexes in rats

作　　者：李迎 崔晓霞[1] 尚世臣[1] 赵权[2] 李桂军[1] 王冬平[1] 陈振文[3] 

机构地区：[1]军事医学科学院实验动物中心,北京100071 [2]吉林农业大学动物科学技术学院,长春130118 [3]首都医科大学实验动物科学部,北京100069

出　　处：《中国比较医学杂志》2017年第10期7-9,共3页Chinese Journal of Comparative Medicine

基　　金：国家科技支撑计划课题(2015BA109B01-04);国家自然科学基金项目(3137227)

摘　　要：目的比较血清和血浆两种不同样本和不同测试方法对大鼠血液生化指标测定值、血糖测定值的影响。方法用血糖仪测定来源相同大鼠的全血血糖值,然后对其血清和血浆样本进行常规生化指标检测。结果在血清与血浆样本进行19个常规生化项目检测中,ALB、TP、ALP、CHOL、URIC、GLU、Mg、LD、Ca差异有显著性(P<0.05),在其余10项血清与血浆检测值比较中,差异无显著性(P>0.05)。不同样本和不同方法对血糖的检测结果分析差异有显著性(P<0.05)。结论不同样本和方法对血清与血浆中部分生化项目检测时存在差异,不同方法和样本检测血糖结果也存在差异。Objective To compare the effects of two different samples and different test methods on blood biochemical indexes and blood glucose values in rats. Methods Glucose levels in the serum and plasma samples were detected with a blood glucometer,and the biochemical parameters in the serum and plasma were determined by routine blood biochemistry. The data were statistically compared and analyzed to determine if there are some significant differences.Results Among the 19 biochemical indexes of serum and plasma specimens,nine parameters,i. e.,ALB,TP,ALP,CHOL,URIC,GLU,Mg,LD,Ca showed significant differences（ P〈0. 05）,while the other 10 indexes showed no significant difference（ P〈0. 05）. Different samples and different methods had significant differences in the detection of blood glucose（ P〈0. 05）. Conclusions Different method and samples impact on the detection of blood glucose and some other serum and plasma biochemical indexes.

关 键 词：生化指标 血清 血浆 大鼠 

分 类 号：R-33[医药卫生]