1α,25(OH)_2D_3对大鼠关节软骨细胞润滑素表达的影响  

1α,25(OH)_2D_3 regulates expression of lubricin of chondrocytes in rat articular cartilage

作　　者：牛国栋[1] 李思维[1] 刘忠军[1] 宋纯理[1] 冷慧杰[1] 

机构地区：[1]北京大学第三医院骨科,北京100191

出　　处：《中国比较医学杂志》2017年第10期28-33,39,共7页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(编号:11472017)

摘　　要：目的在细胞水平,探讨1α,25(OH)2D3对软骨细胞润滑素合成和分泌的影响。方法使用炎性因子TNF-α对大鼠关节软骨细胞进行炎症干预,分别添加不同剂量1α,25(OH)2D3至正常及炎症状态下的软骨细胞,使用ELISA及Western Blot方法分别检测细胞上清中和细胞内润滑素分泌表达的变化,从而评估1α,25(OH)2D3对大鼠关节软骨细胞润滑素的调节作用。结果 TNF-α可以明显降低细胞活性,以及细胞内和细胞上清液中润滑素的表达分泌。1α,25(OH)2D3可以升高正常软骨细胞以及炎症状态下软骨细胞的活性,但是不能调节正常软骨细胞上清和胞内润滑素的分泌和表达。对于炎症状态的软骨细胞,1α,25(OH)2D3可以明显提高润滑素在细胞上清及细胞内的分泌表达,并有一定剂量依赖性。结论 1α,25(OH)2D3能够促进炎症状态下的软骨细胞润滑素的表达分泌,从而更好的保护软骨表面。Objective To investigate the effects of vitamin D on synthesis and secretion of lubricin in chondrocytes at the cellular level. Methods Rat articular chondrocytes were stimulated by TNF-α. Normal and inflammatory chondrocytes were treated by different doses of vitamin D respectively. ELISA and Western Blot were used to detect the secretion of lubricin in the supernatant and the synthesis level in the cells. Results TNF-α significantly reduced the activity of both normal chondrocytes and chondrocytes in inflammatory state. TNF-α also significantly reduced the expression of lubricin in the cells and supernatant. 1α,25（ OH） 2D3 increased the activity of both normal chondrocytes and chondrocytes in inflammatory state. 1α,25（ OH） 2D3 significantly elevated the secretion and expression of supernatant and intracellular lubricin only in chondrocytes stimulated by TNF-α in a dose-dependent manner, but not in normal chondrocytes. Conclusions Vitamin D can promote the secretion and expression of lubricin in inflammatory state chondrocytes,which may act as one of the mechanisms of vitamin D protecting the cartilage surface in osteoarthritis.

关 键 词：1α 25(OH)2D3 润滑素 软骨细胞 关节炎 

分 类 号：R-33[医药卫生]