小鼠CD4^+T细胞外泌体分离及鉴定  

Isolation and identification of exosomes of CD4^+T cells in mice

作　　者：张伟鑫[1] 曾仲[1] 

机构地区：[1]昆明医科大学第一附属医院器官移植中心,昆明650000

出　　处：《中国比较医学杂志》2017年第10期60-64,共5页Chinese Journal of Comparative Medicine

基　　金：云南省卫生科技计划基金资助项目(2014NS155)

摘　　要：目的探讨CD4^+T细胞是否具有分泌外泌体的能力,以及CD4^+T细胞外泌体参与炎症及肿瘤免疫过程的可能。方法应用断颈处死的方法取出小鼠的脾脏,用磁珠分选的方法从小鼠脾脏细胞中分选出CD4^+T细胞,并尝试从培养基上清液中提取CD4^+T细胞的外泌体,在透射电镜下观察,后用PCR技术分析miR-23a、miR-214的表达情况。结果在透射电镜下能够观察到提取出的CD4^+T细胞外泌体,且用PCR技术能检测到小鼠CD4^+T细胞外泌体中miR-23a、miR-214这两个炎症及肿瘤相关的miRNA的表达。结论小鼠CD4^+T细胞有分泌外泌体的能力,且为CD4^+T细胞外泌体参与炎症及肿瘤相关过程提供了可能。Objective To explore whether CD4~＋T cells in mice can secrete exosomes and the possibility of exosomes to participate in the inflammation and immune response process. Methods Spleen samples were taken from mice,and CD4~＋T cells were isolated from the spleen tissue using magnetic bead separation method. Exosomes of CD4~＋T cells were extracted from the supernatant and observed under transmission electron microscope. PCR assay was used to detect the expression of miR-23 a and miR-214. Results Exosomes were observed under elecron microscope,and the expression of miR-23 a and miR-214 were detected by PCR assay. Conclusions CD4~＋T cells in mice can secrete exosomes,which offer possibility to participate in the inflammation and immune response.

关 键 词：CD4+T细胞 外泌体 分离 鉴定 MIRNA 

分 类 号：R-33[医药卫生]