果胶多糖可控性降解及果胶寡糖的制备  

Controlled degradation of citrus pectin polysaccharide and preparation of oligogalacturonic acids from pectic acid

作　　者：李健军[1] 许昭[1] 王仲孚[2] 黄琳娟[2] 

机构地区：[1]西安交通大学理学院,西安710049 [2]西北大学生命科学学院,教育部西部资源生物与生物技术重点实验室,西安710069

出　　处：《西北药学杂志》2017年第6期726-730,共5页Northwest Pharmaceutical Journal

基　　金：国家高技术研究发展计划(编号:2007AA10Z338);中央高校基本科研业务费专项资金(编号:xjj2013112);西部资源生物与现代生物技术教育部重点实验室(西北大学)开放基金(编号:ZS14001)

摘　　要：目的建立一套果胶多糖可控性降解和果胶寡糖分离分析的完整方法。方法采用酸水解和生物酶法等不同降解方法对柑橘果胶多糖进行可控性降解,以所得果胶寡糖的聚合度范围及相对含量为指标,确定最优降解条件,对降解所得的果胶寡糖进行分离分析。结果以果胶酸为底物,用果胶酶40℃反应1h,可得聚合度范围1~5的寡聚半乳糖醛酸混合物;经强阴离子交换树脂Dowex-1×4分离、薄层色谱和电喷雾电离质谱检测,得到聚合度范围1~5的单一聚合度的半乳糖醛酸寡糖。结论建立的果胶多糖可控性降解和果胶寡糖分离的分析方法为果胶多糖的系统研究提供了参考。Objective To develop a controlled depolymerization method for degradation of pectin polysaccharide,and a separation method for oligo galacturonic acids（oligo-GalA）with different degree of polymerization.Methods Different pectin hydrolysis procedures（chemical and enzymatic）were carried out.And combined chemical and enzymatic hydrolysis of pectin was also studied.The combined chemical and enzymatic hydrolysis of pectin and enzymatic hydrolysis of pectic acid showed high recovery of oligo galacturonic acids（oligo-GalAs）.Results An efficient method for the preparation of oligo-GalA,up to dp 5,from pectic acid was described.Pectic acid was digested with a commercially available pectinase to yield a mixture of oligo-GalA,which was effectively separated by an ion-exchange chromatography（Dowex-1×4）to obtain pure oligo-GalA of dp 1-5 and identified by TLC and ESIMS.Conclusion The controlled depolymerization method for pectin polysaccharide and the separation method for oligo galacturonic acids are stable and reproducible,which are valuable for the further study on pectin polysaccharide.

关 键 词：果胶 半乳糖醛酸 降解 分离 电喷雾质谱 

分 类 号：R284[医药卫生—中药学]