艾片加工过程中的化学成分变化及L-龙脑的含量测定  

Determination of L-borneol Content of Aipian and Its Chemical Composition Variation during Processing

作　　者：张影波 庞玉新[1,2] 邹纯礼 王凯[1] 胡璇[1] 陈振夏[1] 黄璐琦[2] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所海南省艾纳香工程技术中心,海南儋州571737 [2]中国中医科学院中药资源中心,北京100700 [3]贵州艾源生态药业开发有限公司,贵州罗甸550100

出　　处：《中国现代中药》2017年第10期1443-1447,共5页Modern Chinese Medicine

基　　金：科技部科研院所研发专项资金项目(2013EG134239);中央级公益性科研院所基本科研业务费专项(1630032015020);贵州省重大科技计划(2013-6011)

摘　　要：目的:通过对艾片加工过程中化学成分变化规律的研究,以期为艾片加工过程的标准化提供依据。方法:利用GC-MS对艾片加工中的中产物艾粉、艾油和艾片进行成分分析,并利用GC法对艾片中的L-龙脑进行测定。结果:1)从艾油中共鉴定出L-龙脑、樟脑、蘑菇醇、β-石竹烯、β-蒎烯等48个化学成分;从艾粉中共鉴定出L-龙脑、2'-羟基-4',6'-二甲氧基苯乙酮、1-苯基-1,3,3-三甲基-二氢化茚、罗汉柏烯等11个化学成分;从艾片中鉴定出L-龙脑、樟脑、异龙脑和α-蒎烯4个化学成分;2)13份艾片中的L-龙脑质量浓度为1.73~1.82 mg·m L–1,其相对含量为86.50%~91.00%(变异系数为0.15%)。结论:对比其加工过程发现,在艾片加工过程中,不仅其化学成分组成发生了变化,而且L-龙脑、α-蒎烯等多个化学成分的含量发生了相对变化。此外,对艾片中的L-龙脑测定表明,13份样品中的L-龙脑含量高于《中华人民共和国药典》不低于85%的含量要求。Objective： The chemical composition variation of Aipian process intermediates were analyzed and compared with GC-MS, with the aim to standard and normalize the technology characteristic and technical details of Aipian machining process. Methods： Firstly, the chemical compositions of Aifen, Aiyou and Aipian were analyzed by GC-MS, and then the L-borneol content in Aipian samples was determined by GC. Results： Forty eight chemical ingredients including L-borneol, camphor, morillol, β-caryophyllene, and β-pinene were determined from Aiyou. Eleven chemical ingredients including L-borneol, 1-（2-hydroxy-4, 6-dimethoxyphenyl）-ethanon, 1-phenyl-1, 3, 3-trimethylindane, and thujopsene were determined from Aifen. Four chemical ingredients including L-borneol, camphor, isoborneol and α-pinene were determined from Aipian. The L-borneol content in 13 Aipian samples was ranged from 1.73-1.82 mg · mL-1 , and its relative percentage content ranged from 86. 50%-91.00% （CV 0. 15% ）. Conclusion： The results indicated not only the chemical compositions varied during the Aipian machining process, but also the relative percentage content of L-borneol, α-pinene, and other chemical compositions changed also during the machining process. And the L-borneol content in 13 Aipian samples indicated those Aipian samples meet the quality requirements of Chinese pharmacopoeia.

关 键 词：艾片 艾粉 艾油 气质联用 L-龙脑 艾纳香 

分 类 号：R284[医药卫生—中药学]