CCL21对结直肠癌侵袭及转移的影响  

Influence of CCL21 on the invasion and metastasis of colorectal cancer

作　　者：戎煜明 陈曦[2] 范德军[3] 林绪涛[3] 郑晓彬[2] 周驰[2] 胡拓 邹一丰[2] 

机构地区：[1]中山大学附属肿瘤医院综合科,广州510610 [2]中山大学附属第六医院结直肠肛门外科,广东省结直肠盆底疾病研究重点实验室,广州510655 [3]中山大学附属第六医院消化内镜科,广州510655

出　　处：《中华胃肠外科杂志》2017年第11期1300-1305,共6页Chinese Journal of Gastrointestinal Surgery

基　　金：国家临床重点专科广东省科技计划项目（2014A020212475）;广东省医学科学技术研究基金（A2015001）：广东省中医药管理局（20151158）

摘　　要：目的探讨CCL21对结直肠癌侵袭及转移的影响。方法应用慢病毒感染的方法，构建CCL21高表达结直肠癌细胞系；应用脂质体转染法，构建CCL21低表达结直肠癌细胞系；通过Transwell侵袭实验与迁移实验、划痕愈合实验及成球实验等一系列体外实验，探讨CCL21对结直肠癌细胞侵袭转移能力及干细胞表型的影响。结果实时荧光定量PCR（QPCR）及蛋白免疫印迹证实，CCL21慢病毒感染和小干扰RNA脂质体转染的方法可分别上调和下调结直肠癌细胞中CCL21表达水平；Transwell实验发现，CCL21高表达结直肠癌细胞的侵袭能力与迁移能力都较CCL21低表达的细胞明显降低，划痕愈合实验也发现，CCL21高表达结直肠癌细胞的迁移速度较慢；另外，CCL21高表达的结直肠癌细胞在成球速度及成球密度方面均低于CCL21低表达的细胞系。结论CCL21可抑制结直肠癌细胞的迁移及侵袭能力，并减弱肿瘤细胞的干细胞表型。Objective To investigate the influence of CCL21 on the invasion and metastasis of colorectal cancer （CRC）. Methods CCL21 over-expressing CRC cell line was constructed by lentivirus infection and CCL21 low-expressing CRC cell line was constructed by lipofection. The effects of CCL21 on the invasion and metastasis of CRC cells and the stem cell-like phenotype were investigated by Transwell migration, invasion assay, wound healing assay and sphere formation assay. Results Real- time quantitative PCR and western blot confirmed that the expression of CCL21 was up-regulated by lentiviral transfection and down-regulated by siRNA liposome transfection. In vitro, Transwell assays showed that the invasion and migration in CCL21 over-expressing CRC cells decreased significantly as compared to those of CCL21 low-expressing cells. In wound healing assay, the CCL21 over-expressing CRC cells showed a significantly lower rate of migration. In addition, the sphere formation rate and density of CCL21 over-expressing CRC cells were lower than those with low-expression of CCL21. Conclusion CCL21 can suppress the migration and invasion of CRC cells and weaken their stem cell- like phenotype.

关 键 词：结直肠肿瘤 基因 CCL21 侵袭与转移 干细胞样表型 

分 类 号：R735.34[医药卫生—肿瘤]