X射线照射离体人血建立染色体畸变剂量-效应曲线  

Establishing dose-response curve of chromosome aberration induced by in vitro X-ray irradiation in human peripheral blood

作　　者：孙杰[1] 王恰[1] 黄嘉华 李宏玲[1] 黄建勋[1] 

机构地区：[1]广东省职业病防治院,广东省职业病防治重点实验室,广东广州510300 [2]广东医科大学,524023

出　　处：《中国职业医学》2017年第5期596-599,共4页China Occupational Medicine

基　　金：广东省职业病防治重点实验室(2012A061400007);广东省医学科研基金(A2014076)

摘　　要：目的建立X射线照射离体人血诱导染色体畸变的剂量-效应曲线。方法采用健康男性肘静脉血,经照射剂量为0.00~5.00 Gy、剂量率为0.8 mGy/s的X射线离体照射,以培养开始加秋水仙素法培养细胞,常规制片,经吉姆萨染色后,采用盲法阅片,记录中期细胞的染色体双着丝粒体、多着丝粒体和双着丝粒环及其伴随断片(以下简称"双+环"),拟合"双+环"率与照射剂量的剂量-效应曲线。结果在0.00~5.00 Gy范围内"双+环"率和畸变细胞率均随照射剂量增加而增高(P<0.01)。0.00~1.00 Gy的最佳拟合方程为y=23.22 D2+4.768 D-0.018(P<0.01);0.50~5.00 Gy的最佳拟合方程为y=34.23 D-3.072(P<0.01)。结论所建立的剂量-效应曲线拟合度较好,可为本实验室评估照射量提供参考依据。Objective To establish the dose-response curve of chromosome aberration induced by X-ray irradiation in human peripheral blood in vitro. Methods The median cubital vein blood of healthy male donors were collected and irradiated with X-ray at the dose of 0. 00-5. 00 Gy in vitro. The dose rate was 0. 8 mGy/s. The cells were cultured with colchicine and stained with routine Giemsa staining. The slices were blindly examined. The morphology of chromosomes were recorded as dicentric,multi-centric,dicentric plus rings or fragments（ hereinafter referred to as dicentric ＋ ring） in metaphase cells. The occurrence of dicentric ＋ ring and the irradiation dose was used to create the dose-response curve.Results The results showed that the occurrence of dicentric ＋ ring in abnormal cells increased with the increasing irradiation dose in the range of 0. 00-5. 00 Gy（ P〈0. 01）. The best fitting equation of 0. 00-1. 00 Gy is y = 23. 22 D2＋4. 768 D-0. 018（ P〈0. 01）. The best fitting equation of 0. 50-5. 00 Gy is y = 34. 23 D-3. 072（ P〈0. 01）.Conclusion The fitting degree of dose-response curve is good,which can be used as reference in laboratory to assess irradiation exposure dose.

关 键 词：X射线 染色体畸变 剂量-效应曲线 

分 类 号：R144.1[医药卫生—公共卫生与预防医学]