羧基化单壁碳纳米管对小鼠CIK细胞表型及功能的影响研究  

Effects on phenotype and function of mouse CIK cells treated with carboxylated single-walled carbon nanotubes

作　　者：王英泽[1] 王巧兰 杜朝[1] 曹晴晴 付育[1] 

机构地区：[1]河北科技大学生物科学与工程学院,石家庄050018

出　　处：《中国免疫学杂志》2017年第11期1616-1620,1625,共6页Chinese Journal of Immunology

基　　金：国家自然科学基金面上项目(81171455);国家自然科学基金青年项目(31100715;81402305);河北省自然科学基金青年项目(C2013208005);河北省高等学校科学技术研究项目(QN2014043);河北科技大学五大平台开放基金课题资助

摘　　要：目的:探讨羧基化单壁碳纳米管(SWCNT-COOH)对小鼠脾脏淋巴细胞诱导而成的CIK细胞表型及功能的影响。方法:以不同浓度的SWCNT-COOH处理CIK细胞,采用倒置荧光显微镜观察细胞生长状态,MTS法测定杀伤效果,流式细胞术分析免疫表型。结果:随着效靶比的增加,CIK的杀伤效果逐渐增强且最适效靶比为20∶1。用0.5μg/ml SWCNTCOOH处理CIK细胞,CD3^+CD4^+、CD3^+CD8^+比例最高且均显著高于对照组(P<0.05),同时CIK杀伤小鼠黑色素瘤细胞B16、小鼠肝癌细胞H22和小鼠前列腺癌细胞RM-1的效果最佳。结论:SWCNT-COOH可以显著增强CIK细胞的体外杀伤活性,具有潜在的肿瘤药物开发价值。Objective：To investigate the effect on the phenotype and function of CIK cells induced from splenic lymphocyte in mice treated with carboxylated single-walled carbon nanotubes.Methods：CIK cells were treated with carboxylated single-walled carbon nanotubes at different concentrations.Cell growth status was observed using inverted fluorescence microscopy.The killing effect was determined by MTS method.Immunophenotype was analyzed by flow cytometry.Results：With the increase of the effect target ratio,the killer rate of CIK was gradually enhanced and the optimal effect target ratio was 20∶1.With the increase of the dose of carboxylated single-walled carbon nanotubes,the proportion of Treg cells decreased.When CIK cells were treated with 0.5 μg/ml carboxylated singlewalled carbon nanotubes（CNTs）,the proportion of CD3~＋CD4~＋ and CD3~＋CD8~＋ was significantly higher than that of the control group（P0.05）,and the killing effects of CIK anchieved best results to B16 cells,H22 cells and RM-1 cells.Conclusion：The carboxylated single-walled carbon nanotubes enhanced the ability of CIK cells to kill tumor cells,significantly which provide has potential value in tumor drug development.

关 键 词：SWCNT-COOH CIK细胞 肿瘤免疫 免疫表型 

分 类 号：R730.51[医药卫生—肿瘤]