Wnt信号通路在姜黄素诱导乳腺癌MCF-7细胞凋亡中作用机制  

Role of Wnt Signaling Pathway in Curcumin-induced Apoptosis of Breast Cancer Cells MCF-7

作　　者：赵轶峰[1] 杨永江[1] 王晓元[1] 梁晚平[1] 李秀娟[1] 黄迪[1] 高晓斌[1] 魏玉磊[1] 

机构地区：[1]河北北方学院附属第一医院,075061

出　　处：《实用癌症杂志》2017年第2期188-191,195,共5页The Practical Journal of Cancer

基　　金：河北省卫生厅科研基金项目(编号:20150468)

摘　　要：目的探讨Wnt通路在姜黄素诱导人乳腺癌MCF-7细胞凋亡中的作用机制。方法将MCF-7细胞培养液随机分为5组,分别加入15、30、60、120μmol/L的姜黄素,对照组不加,培养12,24,48 h后采用CCK-8法检测细胞增殖能力。分A、B两组(剂量组、时间组)采用Annexin V-FITC/PI双染流式细胞术(FCM)观测细胞凋亡及细胞周期分布;Western Blotting法检测A、B两组的Wnt通路相关蛋白,并进行相应的相关性分析探讨Wnt通路与乳腺癌MCF-7细胞凋亡的关系。结果 CCK-8结果显示,随培养时间延长,姜黄素各浓度组MCF-7细胞增殖抑制率逐渐增高(均P<0.05);在同一时间点,随着姜黄素浓度升高,细胞增殖抑制率逐渐增高(均P<0.05)。FCM染色结果显示,随着姜黄素浓度增加、作用时间延长MCF-7细胞凋亡指数逐渐上升(均P<0.05),且G_1/S期细胞增加越多(P<0.05)。Western Blotting实验结果显示MCF-7细胞中Wnt1、β-catenin的表达下调程度呈现剂量时间依赖性(P<0.05)。相关性分析显示细胞抑制率、G_0/G_1期细胞百分比、细胞凋亡率均与Wnt通路相关蛋白Wnt1、β-catenin的表达呈现较明显的相关性(P<0.05)。结论姜黄素的抗癌活性与抗增殖能力具有明显的剂量时间依赖性,且Wnt通路在姜黄素诱导乳腺癌MCF-7细胞抑制、凋亡过程中发挥了重要作用。Objective To investigate the mechanism of Wnt pathway in curcumin-induced apoptosis of human breast cancer cells MCF-7.Methods The MCF-7 cells were randomly divided into 5 groups,respectively received 15,30,60 and 120μmol/L curcumin,and the control group,CCK-8 cells were cultured for 12,24 and 48 hours to detect proliferative capacity . Apoptosis and cell cycle distribution were observed by Annexin V-FITC/PI double staining flow cytometry （FCM）.The Wnt path-way of group A and group B was detected by western blotting method .And to explore the relationship between the Wnt pathway and the apoptosis of breast cancer MCF-7 cells.Results CCK-8 showed that the proliferation inhibition rate of MCF-7 cells in-creased gradually with the increase of curcumin concentration （P〈0.05）;at the same time point,with the increase of curcumin concentration,the inhibition of cell proliferation （P〈0.05）.FCM staining showed that the apoptotic index of MCF-7 cells in-creased gradually with the increase of curcumin concentration （P〈0.05） and the increase of G1/S phase cells （P〈0.05）. Western blotting results showed that the expression of Wnt 1 and β-catenin in MCF-7 cells was dose and time dependent （ P〈0.05）.The correlation analysis showed that the percentage of cells ,G0/G1 phase, apoptosis rate were closely correlated with the expression of Wnt1 andβ-catenin （P〈0.05）.Conclusion The anticancer activity and antiproliferative ability of curcumin have a dose and time dependent manner ,and Wnt pathway has a significant effect on the expression of Wnt 1 and β-catenin in curcu-min-induced breast cancer MCF-7 cells,and it plays an important role in the process of apoptosis .

关 键 词：WNT信号通路 姜黄素 MCF-7细胞 细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤]