MCM7、Rb、c-MYC在肝细胞癌中的表达及其在基因通路中的作用  

Expression of MCM7,Rb,c-MYC Protein in Hepatocellular Carcinoma Tissues and Its Effect on Gene Passageway

作　　者：牛广旭[1] 吴士茜[1] 刘志权[2] 刘小慧 田云霄[1] 

机构地区：[1]河北省邯郸市中心医院,056001 [2]河北省石家庄市第五医院,050021

出　　处：《实用癌症杂志》2017年第2期210-213,共4页The Practical Journal of Cancer

摘　　要：目的探讨MCM7、Rb、c-MYC等基因在肝细胞癌中的表达及其在基因通路中的意义。方法选取2013年1月至2015年8月邯郸市中心医院手术切除的60例原发性肝细胞癌组织进行免疫组化染色,分析MCM7、Rb、c-MYC等基因在肝细胞癌中的表达情况。结果 MCM7蛋白仅在肝细胞癌组织(73.3%)中表达,显著高于正常肝组织和肝硬化组织(P<0.05);Rb蛋白在肝硬化组织(53.3%)和肝细胞癌组织(58.3%)中表达,两者阳性率之间无统计学差异(P>0.05);肝细胞癌组织(70.0%)中c-MYC蛋白阳性率显著高于肝硬化组织(35.0%)(P<0.05)。MCM7蛋白的表达与肿瘤直径、AFP、组织分化程度和临床分期有关(P<0.05),而与病灶数、是否转移及HBs Ag是否阳性无关(P>0.05);Rb蛋白的表达仅与肿瘤组织分化程度有关(P<0.05);c-MYC蛋白表达与肿瘤组织分化程度和是否转移有关(P<0.05)。MCM7蛋白和Rb阳性率呈显著负相关(γ=-0.712,P=0.038;γ=-0.664,P=0.018)。结论肝细胞癌的发生是多基因多通路相互作用的结果,多种调控蛋白的异常共同导致肿瘤的发生。Objective To investigate the expression of MCM7,Rb,c-MYC protein in hepatocellular carcinoma tissues and its effect on gene passageway .Methods Tumor tissues of 60 cases of hepatocellular carcinoma were performed immunohisto-chemistry stain,the expression of MCM7,Rb and c-MYC genes in hepatocellular carcinoma were analyzed .Results MCM7 pro-tein expressed only in HCC tissue（73.3%）,which was significantly higher than normal liver tissues and liver cirrhosis tissues （P〈0.05）.Rb protein expressed in liver cirrhosis （53.3%） and HCC tissues（58.3%）,the positive rates between the 2 tissues had no statistical difference（P〉0.05）.Positive rate of c-MYC protein in HCC tissue（70.0%） was significantly higher than that in liver cirrhosis tissues（35.0%）（P〈0.05）.The expression of MCM7 was related to tumor size,AFP,differentiation degree and clinical stage（P〈0.05）,and not related to tumor foci,metastasis and HBsAg（P〉0.05）.The expression of Rb was related to differentiation degree only（P〈0.05）.The expression of c-MYC was related to differentiation degree and metastasis （P〈0.05）. The positive rate of MCM7 was negatively correlated to that of Rb protein （γ=-0.712,P=0.038;γ=-0.664,P=0.018）. Conclusion The occurrence of hepatocellular carcinoma is the consequence of polygene and multichannel .Anomaly of multiple protein co-induce the occurrence of hepatocellular carcinoma .

关 键 词：肝细胞癌 MCM7蛋白 RB蛋白 C-MYC蛋白 

分 类 号：R735.7[医药卫生—肿瘤]