七氟烷预处理抑制缺氧诱导的脑损伤  

Sevoflurane preconditioning inhibits brain injury in hypoxic mice

作　　者：汪梦霞[1] 李健玲[1] 梁赵佳[1] 钟曌 胡冬华[1] 王菲菲[1] 田和[1] 高兰[1] 李雅兰[1] 

机构地区：[1]暨南大学附属第一医院麻醉科,广东广州510632

出　　处：《中国病理生理杂志》2017年第11期1932-1937,共6页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目(No.2017A30313514);围术期口服多功能液体的研发及其临床前研究(No.2017ZC0012)

摘　　要：目的:观察七氟烷预处理对缺氧小鼠脑损伤的影响及其可能机制。方法:将60只雄性C57BL/6J小鼠,随机分为对照(C)组、缺氧(H)组、2%七氟烷预处理30 min组(S1+H组)、2%七氟烷预处理60 min组(S2+H组)和4%七氟烷预处理30 min组(S3+H组),每组10只。缺氧即持续吸入O2体积分数为(6.5±0.1)%的氮氧混合气体24 h构建缺氧模型;预处理即以O2体积分数为(21.0±0.5)%的氮氧混合气体为载气,分别吸入2%七氟烷30 min、2%七氟烷60 min和4%七氟烷30 min,洗脱15 min后进行缺氧处理。用光学显微镜及透射电子显微镜(TEM)观察海马CA1区形态学改变;比色法检测血清乳酸脱氢酶(LDH)活性;ELISA测定脑组织促红细胞生成素(EPO)和血管内皮生长因子(VEGF)含量;同时测定脑组织丙二醛(MDA)含量及超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPx)活性。结果:缺氧24 h后,光镜下可见海马CA1区细胞水肿或固缩;各预处理组病理改变轻于H组。TEM下S2+H组细胞超微结构最为完整。H组血清LDH活性及脑组织EPO、VEGF、MDA含量显著高于C组,脑组织的SOD及GPx活性较C组明显降低。七氟烷预处理后血清LDH活性及脑组织EPO、VEGF含量较H组降低,以S2+H组最为显著;脑组织MDA含量及SOD活性降低,而GPx活性有所升高。结论:七氟烷预处理能减轻缺氧引起的脑组织损伤,其机制可能与调节抗缺氧蛋白合成及降低氧化应激有关。AIM：To observe the effects of sevoflurane preconditioning on brain injury in hypoxic mice and its possible mechanism.METHODS：Male C57 BL/6 J mice were randomly divided into control（C） group,hypoxia（H）group,2% sevoflurane preconditioning for 30 min ＋ hypoxia（S1 ＋ H） group,2% sevoflurane preconditioning for 60 min＋ hypoxia（S2 ＋ H） group and 4% sevoflurane preconditioning for 30 min ＋ hypoxia（S3 ＋ H） group.The hypoxia model was established by continuous inhalation of（6.5 ± 0.1） % O2 for 24 h.The sevoflurane preconditioning treatments,S1,S2 and S3,were conducted by inhalation of 2% sevoflurane for 30 min,2% sevoflurane for 60 min and 4% sevoflurane for 30 min,respectively,with the carrier of（21.0 ± 0.5） % O2,followed by washout for 15 min and then hypoxia treatment.The histological changes of the hippocampal CA1 area were observed under light microscope and transmission electron microscope（TEM）,and serum lactate dehydrogenase（LDH） activity was measured by colorimetric method.Furthermore,the protein levels of erythropoietin（EPO） and vascular endothelial growth factor（VEGF） in brain tissue homogenate were examined by ELISA,and the content of malondialdehyde（MDA） and the activity of superoxide dismutase（SOD） and glutathione peroxidase（GPx） were measured by microplate reader.RESULTS：After hypoxia for 24 h,cell edema or pyknosis in the hippocampal CA1 area was observed in H group.Sevoflurane preconditioning reduced hypoxic injury,and the cell ultrastructure under TEM was significantly improved in S2 ＋ H group.Compared with C group,the serum LDH activity and the levels of EPO,VEGF and MDA in brain tissues were significantly increased in H group,while the activity of SOD and GPx decreased.After sevoflurane pretreatment,the serum LDH activity and the levels of EPO and VEGF in brain tissues were lower than those in H group,and the most significant difference was observed in S2 ＋ H group.Moreover,the MDA content and SOD activity decreased,and the G

关 键 词：七氟烷 预处理 缺氧 脑损伤 氧化应激 

分 类 号：R363[医药卫生—病理学] R331.1[医药卫生—基础医学]