补阳还五汤促进内皮祖细胞修复损伤血管内皮  

BYHWD promotes endothelial progenitor cells to repair damaged vascular endothelium

作　　者：张伟 贺冰[2] 李亮 李菲[3] 曹浪[3] 刘晓丹[3] 邓常清[3] 

机构地区：[1]湖南中医药大学附属岳阳医院,湖南岳阳414000 [2]湖南中医药大学第二附属医院,湖南长沙410005 [3]湖南中医药大学中西医结合学院,湖南长沙410208

出　　处：《中国病理生理杂志》2017年第11期1969-1974,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81001494);湖南省教育厅重点实验室开放基金项目(No.15k059);湖南省自然科学基金资助项目(No.2016JJ4069)

摘　　要：目的:探讨补阳还五汤(BYHWD)促进内皮祖细胞(EPCs)修复损伤血管内皮的作用及相关机制。方法:以补阳还五汤灌胃及EPCs尾静脉输注作用于内皮损伤的模型大鼠,从血管内皮的形态、功能和EPCs归巢等方面评价内皮损伤修复情况;从血管内环境改善和基质细胞衍生因子-1(SDF-1)及其受体趋化因子受体-4相关蛋白表达了解该方促进EPCs修复受损血管的机制。结果:与单用EPCs组和单用BYHWD组比较,BYHWD联合EPCs组内膜厚度明显减少,甘油三酯、总胆固醇及血钙含量降低,高密度脂蛋白含量升高;此外,BYHWD联合EPCs组血管内皮型一氧化氮合酶(e NOS)及血管SDF-1蛋白表达均显著增加。结论:补阳还五汤能促进EPCs修复损伤的血管内皮,其作用机制可能与调控内环境以及促进EPCs的归巢有关。AIM：To investigate the role of Buyanghuanwu decoction（BYHWD） in promoting endothelial progenitor cells（EPCs）-induced recovery of damaged vascular endothelium.METHODS：The endothelial damaged rats were lavaged with BYHWD and injected with EPCs through vena caudalis.The repaired situation of damaged endothelium was observed.RESULTS：Compared with EPCs group and BYHWD group,the endothelial thickness was reduced,the levels of calcium,triglycerides and total cholesterol were decreased,but the high density lipoprotein levels were increased.In addition,the protein expression of vascular endothelial nitric oxide synthase and vascular stromal cell-drived factor-1 was significantly increased,but the expression of CXC chemokine receptor-4 was significantly reduced in BYHWD ＋ EPC group.CONCLUSION：BYHWD promotes EPCs repairing damaged endothelium,the mechanism may be related to improve the internal environment and promotes the EPCs homing.

关 键 词：补阳还五汤 内皮祖细胞 血管内皮 内皮型一氧化氮合酶 基质细胞衍生因子-1 趋化因子受体-4 

分 类 号：R363[医药卫生—病理学] R285.5[医药卫生—基础医学]