盐析冻融法提取肝炎患者血浆冷沉淀的初步研究  

Preliminary study on salting-out and frozen-thaw method for exracting cryoprecipitate from plasma in hepatitis patients

作　　者：徐兵 黄素钦 吴林岚[2] 杨晓梅 吴开木[2] 

机构地区：[1]福建医科大学孟超肝胆医院肝病研究所,福州350025 [2]福建省第二人民医院检验科 [3]福州市菁华崇辉医院内科

出　　处：《肝脏》2017年第11期1005-1007,共3页Chinese Hepatology

基　　金：福建省医学创新课题项目基金(2017-CXB-12)

摘　　要：目的改进血浆冷沉淀提取方法,并用于提取患者血浆冷沉淀用于回输治疗。方法取重型肝炎患者血浆200 mL,加入与血浆等量的无菌半饱和NaCl溶液,置-30℃冰冻48 h以上(或-80℃,6 h)。然后将冰冻血浆置4℃水浴溶化,4℃下500×g,离心10 min,弃上清留沉淀;在沉淀物中加ACD液重溶至20 mL,即为冷沉淀溶液。检测血浆和相应冷沉淀溶液中Na^+、Cl^-、纤维蛋白原(FIG)、纤维连接蛋白(Fn)、Ⅷ因子、PT、ATPP值。结果冷沉淀溶液中Na^+、Cl^-浓度比血浆中高2~3倍;FIG、Fn和Ⅷ因子含量分别比血浆中高7倍多和近9倍;冷沉淀溶液的PT、ATPP值均比血浆的值降低,但差异无统计学意义(P>0.05)。结论盐析冻融法可用于提取肝炎患者血浆冷沉淀,但提取的冷沉淀需经适当稀释才可用于回输。Objective To investigate the efficiency of salting-out and frozen-thaw method for extracting cryoprecipitate from plasma in hepatitis patients for retransfusion therapy.Methods A total of 200 mL plasma from patients with severe hepatitis were diluted with equal volume of half-saturated sterile NaCl solution.Then the diluted plasma was frozen at-30 ℃for at least 48 h or at-80℃for 6 h.At-4℃,the frozen plasma was thawed and centrifugated at 500 g for 10 min.Removing the supernatant,the sediment was dissolved with acid citrate dextrose(ACD)solution to 20 mL cryoprecipitate(CP)solution.The values of Na^+,Cl^-,fibrinogen(FIG),fibronectin(Fn),factor Ⅷ,prothrombin time(PT)and activated partial thromboplastin time(APTT)of the plasma and CP solution were measured,respectively.Results Compared with plasma,CP solution showed 2~3 times higher levels of Na^+ and Cl^-,7 times higher levels of FIG and Fn,and nearly 9 times higher level of factorⅧ.However,CP solution showed lower levels of PT and ATPP than the plasma,with no significant difference(P>0.05).Conclusion The salting-out and frozen-thaw method could be used to extract CP from plasma,and the extracted CP should be diluted properly for further retransfusion.

关 键 词：冷沉淀 肝炎 盐析 回输 

分 类 号：R575.1[医药卫生—消化系统]