细鳞鲑幼鱼n-3 HUFA需求量的研究  

Requirement of dietary n-3 highly unsaturated fatty acids for juvenile Brachymystax lenok

作　　者：常杰[1] 牛化欣[1] 胡宗福[1] 于建华[1] 李树国[1] 

机构地区：[1]内蒙古民族大学动物科学技术学院,内蒙古通辽028000

出　　处：《淡水渔业》2017年第6期81-87,共7页Freshwater Fisheries

基　　金：国家自然科学基金(31260638;31360640和31460692);内蒙古自治区高等学校"青年科技英才资助计划"项目(NJYT-14-B13)

摘　　要：为确定细鳞鲑(Brachymystax lenok)n-3 HUFA需求量以减少鱼油使用和降低养殖成本,研究饲料中不同水平的n-3 HUFA对细鳞鲑的生长性能、体成分和肌肉脂肪酸组成的影响。以脱脂鱼粉、脱脂豆粕、明胶和酪蛋白为主要蛋白源,通过调节饲料中的猪油和浓缩油EPA、DHA水平,使饲料n-3HUFA的含量分别达到0.25%、0.50%、0.75%、1.00%、1.25%、1.50%,配制出6种等氮等能的试验饲料(D 0.25、D 0.50、D 0.75、D1.00、D 1.25和D 1.50),分别投喂细鳞鲑幼鱼(60.0 g±2.8 g)84 d。结果显示:饲料中n-3HUFA不同水平对细鳞鲑成活率和饲料系数没有显著影响,但是显著影响了其末重(FW)、增重率(WGR)和特定生长率(SGR)。随着添加饲料中n-3HUFA水平的升高,FW、WGR和SGR有先升高后下降的趋势,且3者在饲料中n-3HUFA水平为0.75%均最大。随着饲料中n-3 HUFA水平的升高,鱼肌肉18∶1n-9的含量逐渐下降,而22∶6n-3的水平相应升高。结果表明,以WGR为评价指标时,用二次曲线模型推测出细鳞鲑对饲料n-3 HUFA的需求量约为0.69%。In order to determine the demand of n-3 highly unsaturated fatty acids （ HUFA） ,to reduce fisli oil content in feed and reduce the cost of culture, the experiment was evaluated to the effects of d ife re n t dietary levels n-3 HUFA on growth performance,body composition and fatty acid composition of muscle. The content of n-3HUFA in feed were 0. 25,0. 50,0. 75, 1. 00 and 1. 25% by adjusting the contents of EPA and DHA in feedstuff and defatted fishmeal,defattedsoybean meal, gelatin and casein as the main protein source （ D 0. 25, D 0. 5 0 ,D 0. 7 5, D 1 .0 0, D 1 .2 5, and D 1. 50） . Fish were fed on six experimental feeds （60. 0 ±2. 8 g） for 84 d. The results showed that n -3HUFAh ad no sig-nificant effect on survival rate and feed conversion rate （〈〉 0. 05 ）, but had significant effects on final weight （ FW ） , weight gain rate （ WGR） and specific growth rate （SGR）. FW,WGR and SGR increased firstly and thing dietary n-3 HUFA levels, and maximum value of FW,WGR and SGR were obtain at D 0. 75 group. The content of muscle 18： 1n -9 gradually decreased,while the level of 22- 6n-3 increased accordingly. In summary, WGR as an evalua-tion index, the requirement for n-3 HUFA of trout is about 0. 69% of diet by8i8.

关 键 词：细鳞鲑(Brachymystax lenok) n-3 HUFA 脂肪酸组成 营养需求量 

分 类 号：S963.16[农业科学—水产养殖]