TAGLN基因对膀胱癌患者的临床意义:基于GEO数据集的分析  

Clinical significance of TAGLN expression in bladder cancer:an analysis based on the GEO database

作　　者：尹晓红 刘小平 罗先武 孟详喻 曾宪涛 王行环[1,3] 

机构地区：[1]武汉大学中南医院循证与转化医学中心.武汉大学循证与转化医学中心,湖北武汉430071 [2]武汉大学健康学院护理学系,湖北武汉430071 [3]武汉大学中南医院泌尿外科,湖北武汉430071

出　　处：《现代泌尿外科杂志》2017年第11期818-822,共5页Journal of Modern Urology

基　　金：国家重点研发计划专项基金(No.2016YFC0106300)

摘　　要：目的探讨TAGLN基因在膀胱癌患者中的表达情况及与临床病理特征的关系,评价其对膀胱癌患者临床预后的影响。方法检索并下载GEO膀胱癌基因表达数据集,分析膀胱癌患者基因表达谱数据及临床信息,明确TAGLN基因对膀胱癌患者预后的影响,并通过基因富集分析方法进一步探讨TAGLN基因对膀胱癌细胞的具体作用机制。结果膀胱癌患者中TAGLN低表达组膀胱癌患者在肿瘤侵袭程度及T、N、M分期方面明显优于TAGLN高表达组膀胱癌患者(P=0.001,P=0.002,P=0.009,P=0.05);而在年龄、肿瘤恶性等级和肿瘤是否进展的分布方面无明显差异;TAGLN低表达组患者的肿瘤特异性生存期(P<0.000 1)和总生存期(P<0.000 1)亦均优于TAGLN高表达组的患者,提示低表达组患者预后更佳。TAGLN基因高表达组的膀胱癌样本主要富集在Notch信号通路、TGF-β信号通路、上皮-间质转换、缺氧、炎性应答、TNF-αNF-κB信号通路等多种生物学过程。这些通路主要与肿瘤血管细胞生成和肿瘤细胞分化、侵袭和转移相关。结论 TAGLN基因与膀胱癌多个病理指标相关,可以作为潜在的诊疗膀胱癌患者预后的标志物和治疗肿瘤的靶标。Objective To explore the relationship between TAGLN expression and clinical prognosis of bladder cancer.Methods Bladder cancer gene expression profiles GEO was downloaded and retrieved.The relationship between TAGLN expression and prognosis of bladder cancer patients was analyzed.Gene set enrichment analysis(GSEA)was applied to investigate the exact mechanisms regarding TAGLN expression and the proliferation of bladder cancer cells.Results Bladder cancer patients with higher TAGLN expression were associated with better TNM staging(P=0.002,P=0.009,and P=0.05,respectively),invasiveness(P=0.001),progression-free survival(P<0.0001),and overall survival(P<0.0001)compared with those with lower TAGLN expression.The results of GSEA indicated that TAGLN might promote the proliferation of bladder cancer cells through biological processes involving Notch signaling,TGF-βsignaling,epithelial mesenchymal transition,hypoxia,inflammatory response and TNF-αsignaling.Conclusion Our results suggested that TAGLN might be an independent risk factor for patients with bladder cancer.

关 键 词：TAGLN基因 膀胱癌 临床预后 GSEA分析 GEO数据库 

分 类 号：R737.14[医药卫生—肿瘤]