二甲双胍联合卡铂对三阴性乳腺癌细胞增殖的影响  

Effects of Metformin combined with Carboplatin on the Proliferation of Triple Negative Breast Cancer Cells

作　　者：赵晓红[1] 冯丽[1] 张百红[1] 马文英 胡文梅 

机构地区：[1]兰州军区兰州总医院肿瘤科,甘肃兰州730000

出　　处：《现代生物医学进展》2017年第30期5837-5839,5848,共4页Progress in Modern Biomedicine

基　　金：甘肃省自然科学基金项目(1308RJZA181)

摘　　要：目的:探讨二甲双胍联合卡铂对三阴性乳腺癌细胞MDA-MB-231增殖的影响。方法:体外培养三阴性乳腺癌细胞MDA-MB-231,分别给予不同浓度(0、2.5、5、10 mmol/L)二甲双胍和20μmol/L卡铂处理后,采用MTT实验、平板克隆实验检测二甲双胍联合卡铂对MDA-MB-231细胞增殖的影响。结果:MTT实验结果显示:二甲双胍抑制MDA-MB-231细胞的增殖,5、10mM组细胞OD490值均显著低于对照组(P<0.05),且10 mM组细胞OD490值明显低于其他各组(P<0.05);与单药相比,二甲双胍和卡铂联用组细胞生长抑制率显著升高,差异具有统计学意义(P<0.05)。平板克隆实验结果显示:与单药相比,二甲双胍和卡铂联用组细胞的克隆形成抑制率率显著升高,差异具有统计学意义(P<0.05)。结论:二甲双胍联合卡铂能够有效的抑制三阴性乳腺癌细胞MDA-MB-231的增殖。Objective： To explore the effect of metformin combined with carboplatin on the proliferation of triple negative breast cancer cells MDA-MB-231. Methods： MDA-MB-231 cell was cultured in vitro and treated with different concentrations （0, 2.5, 5, 10 mmol/L） of metformin and 20 p, mol/L carboplatin, respectively. MTT and clone formation assays were used to detect the effect of metformin combined with carboplatin on the proliferation of MDA-MB-231 cells. Results： MTT assay showed that metformin inhibited the proliferation of MDA-MB-231 cells. The cells OD490 values in 5 or 10mM group were significantly lower than that of the control group （P 〈0.05）, and the OD490 values in 10 mM group were significantly lower than those in the other groups （P 〈0.05）. Compared with monotherapy, the cell growth inhibition rate of metformin combined with carboplatin was significantly increased （P〈0.05）. The results of plate clone formation assay showed that compared with monotherapy, the cell clone formation inhibition rate of metformin combined with carboplatin was significantly increased （P〈0.05）. Conclusion： Metformin combined with carboplatin could effectively reduce the proliferation of triple negative breast cancer cell line MDA-MB-231.

关 键 词：二甲双胍 卡铂 三阴性乳腺癌细胞 细胞增殖 

分 类 号：R-33[医药卫生] R737.9