山萘酚对人非小细胞肺癌细胞系A549增殖的影响  

The Effect of Kaempferol on the Proliferation of Human Alveolar Adenocarcinoma Cell Line A549

作　　者：左一雄 蔡湘仪 吴彦萍[1,2] 黄来强 

机构地区：[1]清华大学生命科学学院,北京100084 [2]深圳市基因与抗体治疗重点实验室和生物医药研究中心清华大学深圳研究生院生命与健康学部,广东深圳518055

出　　处：《现代生物医学进展》2017年第31期6001-6005,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30570960);深圳市科技计划项目(JCYJ20140418112611757;GJHZ20140416153844269)

摘　　要：目的:探究山萘酚(Kaempferol,Kpf)对非小细胞肺癌细胞系A549增殖的影响。方法:采用不同浓度(0、5、10和20μg·m L-1)的山萘酚处理体外培养的A549细胞后,通过四氮唑蓝(MTT)试验和平板克隆试验探究山萘酚对A549增殖的影响,实时定量聚合酶链式反应(Q-PCR)来探究山萘酚作用下rDNA的转录情况,免疫印迹试验探究山萘酚对上游结合因子(Upstream binding factor,UBF)磷酸化的影响。结果:MTT和平板克隆试验证明山萘酚对A549细胞的增殖有较强的抑制作用,存在剂量依赖关系。Q-PCR证明了山萘酚能够下调rDNA的转录。通过免疫印迹试验能够证明山萘酚可以降低UBF磷酸化的水平。结论:山萘酚对人非小细胞肺癌细胞系A549的生长有抑制作用,可能与下调UBF的磷酸化水平影响rDNA的转录有关。Objective： To study the effect of Kaempferol on the proliferation of human alveolar adenocarcinoma cell line A549. Methods： A549 cells was treated with different concentrations of Kaempferol（0, 5, 10, 20, 50 μg·mL^-1）. MTT assay and plate cloning experiments were contributed to study the effect of Kaempferol on the cell proliferation. The nucleolus transcription level was detected by real-time polymerase reaction experiment. Western blot was performed to detect the phosphorylation of upstream binding factor （UBF） protein. Results： MTT assay and plate cloning experiments showed that Kaempferol significantly decreased the viability of A549 cells in a dose dependent manner. Moreover, Keampferol down-regulated the level of nucleolus transcription, declined the phosphorylated UBF protein level. Conclusions： Keampferol could inhibit the proliferation of A549 cells, which might be related to the down-regulation of pre-rRNA and p-UBF protein level.

关 键 词：山萘 A549细胞 上游结合因子 增殖 

分 类 号：R-33[医药卫生] R734.2