反义ACC合成酶基因导入番茄的研究  

Studies on transformation of antisense ACCase gene into tomatoes

作　　者：曾黎琼[1] 张绍松[1] 柴红梅[1] 王敬乔[1] 李成云[1] 张云孙[2] 罗云波[3] 

机构地区：[1]云南省农业科学院生物技术研究所,云南昆明650223 [2]云南大学,云南昆明650091 [3]中国农业大学,北京100094

出　　处：《云南大学学报（自然科学版）》2002年第5期393-397,共5页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南省省院省校合作项目资助 (98ZB0 5 )

摘　　要：采用农杆菌介导法将携带有反义ACC合成酶基因和NPTII的表达载体 pKAC导入番茄栽培品种美国大红五星 10 2、美国大红 10 8、美国大红王、大红II号等中 .经过卡那霉素筛选后 ,获得了一批抗性苗 ,其中有 3株PCR检测为阳性 .实验证明 ,不同激素配比对番茄子叶不定芽的诱导有一定影响 ,适宜的农杆菌侵染时间和浓度是番茄基因转化成功与否的关键 。An expressing vector pKAC with antisense ACCase gene and NPT II was transferred into some tomato strains by agrobacterium mediated transformation.After selection culture with kanamycin,some transgenic plants of tomato were obtained.Three of them were positive by PCR analysis.The results showed three characteristics,namely,1.The inductive rate of adventitious bud of tomato cotyledon was different on various media with different hormone and concentration.2.The concentration of agrobacterium and time of agrobacterium infection were the keys to gene transformation of tomato.3.The delayed selection culture was helpful to the growth of transgenic cells.

关 键 词：农杆菌介导法 番茄 反义ACC合成酶基因 延迟选择 转基因植物 基因转化 遗传育种 

分 类 号：S641.2[农业科学—蔬菜学] Q943.2[农业科学—园艺学]