胃癌患者癌组织及血浆中长链非编码RNAXIST的表达与临床意义  

Expression and clinical significance of long non-coding RNA XIST in tissue and plasma of gastric cancer

作　　者：李江 史征[1] 罗天航[1] 聂明明[1] 袁士杰 毕建威[1] 

机构地区：[1]第二军医大学长海医院胃肠外科,上海200433

出　　处：《第二军医大学学报》2017年第11期1403-1409,共7页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(81472277)~~

摘　　要：目的探讨长链非编码RNA XIST(lncRNA-XIST)在胃癌患者癌组织和血浆中的表达水平及其临床意义。方法收集2017年2月至7月于第二军医大学长海医院就诊的40例胃癌患者手术切除的胃癌组织及癌旁组织标本,抽取同期90例胃癌患者的术前血浆样本和90例健康体检者血浆样本。利用实时定量PCR(qPCR)检测上述标本中lncRNA-XIST的表达水平。采用非参数检验分析lncRNA-XIST表达水平与胃癌患者临床病理参数(年龄、性别、TNM分期、肿瘤最大径、淋巴结转移、分化程度、Ki-67阳性率)的关系。通过绘制受试者工作特征(ROC)曲线评价lncRNA-XIST在诊断胃癌时的效能。结果胃癌组织中lncRNA-XIST表达较癌旁组织升高,胃癌患者血浆lncRNAXIST表达高于健康体检者,差异均有统计学意义[0.150(0.094,0.247)vs 0.085(0.041,0.193)、0.189(0.119,0.256)vs 0.144(0.095,0.180),P均<0.05]。胃癌组织及血浆中的lncRNA-XIST表达水平与胃癌TNM分期、淋巴结转移、分化程度相关(癌组织:Z=3.147、2.729、2.393,血浆:Z=2.769、2.431、2.144;P均<0.05)。血浆lncRNAXIST诊断胃癌ROC曲线下面积(AUC)为0.753(95%CI:0.681~0.825,P<0.001),截断(cut-off)值为0.197时灵敏度为51.1%、特异度为95.6%;诊断TNMⅠ~Ⅱ期胃癌AUC为0.694(95%CI:0.592~0.796,P<0.01),灵敏度为38.5%,特异度为95.6%;均高于癌胚抗原(CEA)和糖类抗原(CA)19-9、CA72-4单独及联合检测。结论 lncRNAXIST在胃癌组织及胃癌患者血浆中表达升高,可能是胃癌的一个潜在的肿瘤标志物。Objective To investigate the expression levels of long non-coding RNA XIST(lncRNA-XIST)in cancer tissues and plasma of gastric cancer patients and its clinical significance.Methods Resected tumor tissues and adjacent non-tumor tissues of 40 patients with gastric cancer in Changhai Hospital of Second Military Medical University were collected from Feb.to Jul.2017;the plasma samples were collected from 90 patients with gastric cancer and 90 healthy volunteers.The expression level of lncRNA-XIST in the above samples was detected by real-time quantitative PCR(qPCR).The relationship between the expression level of lncRNA-XIST and the clinicopathological parameters related to gastric cancer(age,gender,TNM stage,tumor maximum diameter,lymph node metastasis,differentiation,and Ki-67 positive rate)were analyzed by nonparametric test.The efficacy of plasma lncRNA-XIST in the diagnosis of gastric cancer was evaluated by plotting the receiver operating characteristic(ROC)curve.Results The expression of lncRNA-XIST in tumor tissues was significantly increased compared with adjacent non-tumor tissues(0.150[0.094,0.247]vs 0.085[0.041,0.193],P<0.05),and the lncRNA-XIST expression in plasma in the gastric cancer group was significantly higher than that in the healthy group(0.189[0.119,0.256]vs 0.144[0.095,0.180],P<0.05).The expression levels of lncRNA-XIST in plasma and cancer tissues of gastric cancer patients were related to TNM staging,lymph node metastasis and differentiation(cancer tissues:Z=3.147,2.729 and 2.393;plasma:Z=2.769,2.431 and2.144;all P<0.05).Area under ROC curve(AUC) performed by plasmatic lncRNA-XIST for gastric cancer was 0.753(95%CI0.681-0.825,P<0.001);when the cut-off value was 0.197,the sensitivity was 51.1%and the specificity was 95.6%.The AUC of diagnosing gastric cancer in TNM Ⅰ-Ⅱ was 0.694(95%CI0.592-0.796,P<0.01),with a sensitivity of 38.5% a the specificity of 95.6%.All values in above two cases were higher than those of carcinoembryonic antigen(CEA),carbohydrate antigen(CA)19-9,CA72-4 alone or co

关 键 词：长链非编码RNA lncRNA-XIST 胃肿瘤 血浆 组织 

分 类 号：R735.2[医药卫生—肿瘤]