地衣活性化合物C_(11)H_(12)O_5体外抗肿瘤作用  

In Vitro Anticancer Effects of Lichen Bioactive Compound C_(11)H_(12)O_5

作　　者：姜重阳 贺小琼 姚乾[2] 税靖霖 

机构地区：[1]昆明医科大学公共卫生学院,云南昆明650500 [2]昆明医科大学第三附属医院云南省肿瘤研究所,云南昆明650031

出　　处：《昆明医科大学学报》2017年第9期7-10,共4页Journal of Kunming Medical University

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项基金重点资助项目(2012FB001);昆明医科大学重大科技成果培育基金资助项目(CGPY201603)

摘　　要：目的研究地衣活性化合物C_(11)H_(12)O_5对人乳腺癌细胞株MCF-7、人肝癌细胞株HepG2及人肺腺癌细胞株A-549的体外抗癌作用,观察其体外抗癌的剂量-效应关系和时间-效应关系.方法采用体外细胞培养方式,以MTT法检测OD值,计算不同处理浓度和处理时间下肿瘤细胞的增殖抑制率.结果化合物C_(11)H_(12)O_5在剂量2、4、8、16和32μg/m L时,对人乳腺癌细胞株MCF-7的抑制率分别为14.19%、13.84%、28.90%、59.50%、80.29%,IC_(50)为12.43μg/m L;对人肝癌细胞株HepG2的抑制率分别为3.89%、11.09%、21.66%、46.27%、66.69%,IC_(50)为18.88μg/m L;人肺腺癌细胞株A-549的抑制率分别为14.13%、11.09%、45.98%、86.19%、99.02%,IC_(50)为6.21μg/m L.结论地衣活性化合物C_(11)H_(12)O_5对人乳腺癌细胞株MCF-7、人肝癌细胞株HepG2、人肺腺癌细胞株A-549均有显著体外抗肿瘤作用,存在明显的剂量-效应关系,其中A-549对该化合物最敏感.对A-549体外抑制作用时间-效应关系不明显.Objectives To investigate the in vitro anticancer effects of lichen bioactive compound C11H1205on human breast cancer cell line MCF-7,human hepatoma cell line HepG2,human lung adenocarcinoma cell lineA-549,and observe the dose- effect and time- effect. Methods MTT assay was used in the in vitro anticancerstudy. The OD values at 24h,48h,72h were examined and the inhibitory ratios were calculated. Results Theinhibitory ratios were 14.19%,13.84%,28.90%,59.50%,80.29% in MCF-7;3.89%,11.09%,21.66%,46.27% and 66.69% in HepG2 and 14.13%,11.09%,45.98%,86.19%,99.02% in A-549 at2, 4, 8, 16,32 μg/mL of compound C11H1205. The IC50 were 12.43 μg/mL,18.88 μg/mL and 6.21 μg/mL in MCF-7,HepG2 and A-549 respectively. Conclusions Compound C11H1205 has significant in vitro anticancer effects onMCF-7,HepG2,A-549. The anticancer effects is dose dependent. A-549 is the most sensitive cell line. The time-effect of the in vitro anticancer effect in A-549 is not evident.

关 键 词：地衣 体外抗癌 MTT MCF-7 HepG2 A-549 

分 类 号：R730[医药卫生—肿瘤]