高剂量电离辐射对恒河猴器官组织病理改变及相关细胞因子表达的影响  

Effects of High Dose Ionizing Radiation on Histopathological Changes and Expression of Related Cytokines in Rhesus Monkeys(Macaca mulatta)

作　　者：高琪[1] 罗启慧[1,2] 曾文 黄超 刘文涛[1] 龚立[3] 陈正礼 

机构地区：[1]四川农业大学动物医学院/实验动物疾病模型研究室,成都611130 [2]四川农业大学实验动物工程技术中心,雅安625014 [3]四川普莱美生物科技集团有限公司,成都610041

出　　处：《农业生物技术学报》2017年第11期1831-1837,共7页Journal of Agricultural Biotechnology

基　　金：国家科技支撑计划(No.2014BAI03B01);国家重大科学仪器设备开发专项(No.2013YQ49085906)

摘　　要：放射治疗已成为治疗肿瘤的一种重要方法,但在治疗同时,高剂量的电离辐射会对机体各个组织器官造成损伤。为探讨高剂量电离辐射对恒河猴(Macaca mulatta)心、肝、脾、肺、肾及肠道组织的病理性损伤及相关细胞因子表达的影响,本研究用辐射能量9.5 Gy的60Coγ对3只雄性恒河猴照射4 h,于辐射后15 d处死并采集相关器官组织,采用苏木精-伊红染色(hematoxylin-eosin staining,HE)观察心、肝、脾、肺、肾及肠道组织病理损伤情况,同时采用免疫组化和图像分析技术检测各组织内肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-6(interleukin-6,IL-6)、IL-10、IL-2和转化生长因子β1(transforming growth factorβ1,TGF-β1)的表达。结果表明,辐射后15 d,跟对照相比,恒河猴心、肝、脾、肺及肾组织发生明显病理变化,而且TNF-α、IL-6、IL-10、IL-2和TGF-β1表达量显著高于对照组(P<0.05),说明电离辐射所致的放射性损伤对相关细胞因子表达有影响,研究结果可为放射性损伤的诊断提供辅助参考。Radiation therapy has become an important method of treating tumors,but at the same time,high doses of ionizing radiation can cause damage to the tissues and organs.To study the effects of high-dose ionizing radiation on the pathological injury and related cytokine expression in the heart,liver,spleen,lung,kidney and intestinal tract of rhesus monkey（Macaca mulatta）,three male rhesus monkeys were irradiated with 9.5 Gy of 60Co γ for 4 h,and the organs were sacrificed 15 days after irradiation.The pathological changes of heart,liver,spleen,lung,kidney and intestinal tissues were observed by hematoxylin-eosin staining（HE）.The expression of tumor necrosis factor-α（TNF-α）,interleukin-6（IL-6）,IL-10,IL-2 and transforming growth factor β1（TGF-β1） in each tissue were detected by immunohistochemistry and image analysis.The results showed that the heart,liver,spleen,lung and kidney tissues of treated rhesus monkeys had obvious pathological changes compared with the control,and the expression of factors was significantly higher than that of the control group（P〈0.05） at 15 days after irradiation.This indicates that radioactive injury caused by ionizing radiation has an effect on the expression of related cytokines.The results of the study provide a supplementary reference for the diagnosis of radioactive injury.

关 键 词：电离辐射 组织病理变化 细胞因子 恒河猴 

分 类 号：S185[农业科学—农业基础科学] R361[医药卫生—病理学]