猪印记基因MKRN3的SNPs筛选及胴体性状关联分析  

SNPs identification of porcine imprinting gene MKRN3 and association analysis with carcass traits

作　　者：汪龙梅[1] 郭玲[1] 刘玉兰[1] 付书林[1] 陈洪波[1] 张晶[1] 邓昌彦[2] 

机构地区：[1]武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023 [2]华中农业大学农业动物遗传育种与繁殖教育部重点实验室,武汉430070

出　　处：《华中农业大学学报》2017年第6期77-82,共6页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(31601922);湖北省自然科学基金项目(2014CFB345)

摘　　要：以梅山猪、大白猪和长白猪为研究对象,筛选出MRKN3基因的单核苷酸多态位点(SNPs)并进行PCR-RFLP验证,利用PCR-Bst UI-RFLP对大白×梅山F2代资源家系(285头)DNA样品酶切分型,将分型结果与猪胴体性状进行关联分析。结果表明:猪MRKN3基因编码区有7个SNPs,分别为293C>G、361G>A、497G>A、630G>A、678T>C、1376C>T、和1407T>A;其中678T>C位点等位基因频率在中外不同猪种中存在差异;猪MRKN3基因678T>C位点与内脂率及臀部膘厚显著相关(P<0.05);678T>C位点对内脂率具有显性效应(P<0.05),表现为该位点杂合子比纯合子具有更高的内脂率;而该位点对臀部膘厚具有加性效应(P<0.05),表现为等位基因678C的累加具有降低臀部膘厚的效应。因此,猪MKRN3基因的678T>C位点可为猪的分子育种实践提供一定指导作用。Single nucleotide poiymorphisms （SNPs） of the porcine imprinting gene MKRN3 were identified in Meishan, Large white and Landrace pigs by PCR-RFLP and were genotyped in the F2 popu- lation of 285 Large white × Meishan pigs by PCR-BstUI-RFLP. Subsequently, correlation between the porcine gene MKRN3 polymorphism and porcine carcass traits were analyzed.The results showed that, the porcine MKRN3 gene had seven SNPs in the coding region, including 293C〉G, 361G〉A, 497G〉A, 630G〉A,678T〉C, 1376C〉T,and 1407T〉A.Of which, the 678T〉C site had different allele frequen- cies among domestic and foreigner pig species.In addition, the 678T〉C site of the porcine MKRN3 was associated with internal fat rate and buttock fat thickness significantly （P〈0.05）.The 678T〉C site had a dominant effect on internal fat rate （P〈0.05）, showing an increased internal fat rate with heterozygote form and had an additive effect on buttock fat thickness （P 〈0.05）, showing a decreased buttock fat thickness with the additive of the 678C allele.Therefore, the 678T〉C site of the porcine MKRN3 might provide a guiding value for the molecular breeding practice.

关 键 词：猪 MKRN3 SNPS 内脂率 臀部膘厚 印记基因 

分 类 号：S828[农业科学—畜牧学]